Using ^^C02, ^^02 and H2O gas exchange as weU as metabolite analysis, net CO2 uptake (P]4) and transpiration rate (£) were measured in the water-stressed plants of Digitalis lanata EHRH. The leaf conductance (gcch). the gross CO2 uptake (Pq), Úie photorespiration (Rp) and reassíinilation (RA) rates were calculated from measuied parameters. The pulse modulated fluorescence was measured duiing the steady statě photosynthesis. After withholding iirigation, the leaf water potential decreased to -2.S MPa, but leaves remained turgid and fully exposed to iiradiance even at a severe water stress. Due to the stress-induced reduction of gcch. and E were drastically reduced, whereas Pq and Rp were less affected. Water use efficiency (WUE), which was higher in 1 000 than 350 cm3(C02) increased as the water stress developed. The stomatal closure induced an increase in the reassimilation (RA) of internally liberated CO2 (Rp). The increased CO2 recycling in relation to the water stress was high in 350 cm^(C02) m-^ and still substantial in 1 000 cm3(C02) and consumed a substantial amount of radiant energy in the form of ATP and reduction equivalents. Consequently, the metabolic demand for radiant energy was reduced by less than 40 %, whereas was diminished by more than 70 % in severely stressed plants at 350 cm3(C02) m*3. Additionally, the quantum efiBciency of photosystem 2 as a measure for the flux of photosynthetically generated electrons was reduced upon the stress. This (and possibly other mechanisms) enabled the stressed plants to avoid overreduction of the photosynthetic electron transport chain.
Computed tomography (CT) is an effective diagnostic modality for three-dimensional imaging of bone structures, including the geometry of their defects. The aim of the study was to create and optimize 3D geometrical and real plastic models of the distal femoral component of the knee with joint surface defects. Input data included CT images of stifle joints in twenty miniature pigs with iatrogenic osteochondrosis-like lesions in medial femoral condyle of the left knee. The animals were examined eight and sixteen weeks after surgery. Philips MX 8000 MX and View workstation were used for scanning parallel plane cross section slices and Cartesian discrete volume creation. On the average, 100 slices were performed in each stifle joint. Slice matrices size was 512 x 512 with slice thickness of 1 mm. Pixel (voxel) size in the slice plane was 0.5 mm (with average accuracy of ± 0.5 mm and typical volume size 512 × 512 × 100 voxels). Three-dimensional processing of CT data and 3D geometrical modelling, using interactive computer graphic system MediTools formerly developed here, consisted of tissue segmentation (raster based method combination and 5 % of manual correction), vectorization by the marching-cubes method, smoothing and decimation. Stifle- joint CT images of three individuals of different body size (small, medium and large) were selected to make the real plastic models of their distal femurs from plaster composite using rapid prototyping technology of Zcorporation. Accuracy of the modeling was ± 0.5 mm. The real plastic models of distal femurs can be used as a template for developing custom made press and fit scaffold implants seeded with mesenchymal stem cells that might be subsequently implanted into iatrogenic joint surface defects for articular cartilage-repair enhancement., P. Krupa, P. Kršek, M. Javorník, O. Dostál, R. Srnec, D. Usvald, P. Proks, H. Kecová, E. Amler, J. Jančář, P. Gál, L. Plánka, A. Nečas., and Obsahuje bibliografii
A comparison of the effects of ultrasound produced by low- and high-frequency ultrasonic apparatuses upon biological systems is one of the basic problems when studying ultrasound cavitation effects. One possibility for how to compare these effects is the indirect method which uses well-known physical quantities characterizing the interaction of ionizing radiation with matter and which also converts these quantities to one common physical quantity. The comparison was performed with two methods applied to the chemical dosimetry of ionizing radiation. The first method employed a twocomponent dosimeter which is composed of 50 % chloroform and 50 % re-distilled water (i.e. Taplin dosimeter). The other method used a modified iodide dosimeter prepared from a 0.5 M potassium iodide solution. After irradiation or ultrasound exposure, measurable chemical changes occurred in both dosimeters. The longer the exposure, the greater the chemical changes. These effects are described by the relationship of these changes versus the exposure times in both dosimeters. The UZD 21 ultrasonic disintegrator (with a frequency of 20 kHz, 50 % power output) was used as a lowfrequency ultrasound source, and the BTL-07 therapeutic instrument (with a frequency of 1 MHz and intensity of 2 W/cm2) was used as a high-frequency cavitation ultrasound source. For comparison, a 60Co gamma source was applied (60Co, gamma energies of 1.17 and 1.33 MeV, activity of 14 PBq). Results of this study have demonstrated that the sonochemical products are generated during exposure in the exposed samples of both dosimeters for all apparatuses used. The amount of these products depends linearly upon the exposure time. The resulting cavitation effects were recalculated to a gray-equivalent dose (the proposed unit is cavitation gray [cavitGy]) based on the sonochemical effects compared to the effects of ionizing radiation from the 60Co source., B. Kratochvíl, V. Mornstein., and Obsahuje bibliografii
Electrogastrography (EGG) is a non-invasive method for the assessment of gastric myoelectrical activity. Porcine EGG is comparable with human one. The purpose of this study was to evaluate the effect of atropine and neostigmine on the EGG in experimental pigs. Adult female pigs were administrated atropine (1.5 mg i.m., n=6) and neostigmine (0.5 mg i.m., n=6) after the baseline EGG, followed by a 90-min trial recording (MMS, Enschede, the Netherlands). Running spectral analysis was used for the evaluation. The results were expressed as dominant frequency of slow waves and EGG power (areas of amplitudes). Neostigmine increased continuously the dominant frequency and decreased significantly the EGG power. Atropine did not change the dominant frequency significantly. However, atropine increased significantly the EGG power (areas of amplitudes) from basal values to the maximum at the 10-20-min interval. After that period, the areas of amplitudes decreased significantly to the lowest values at the 60-90-min interval. In conclusion, cholinergic and anticholinergic agents affect differently EGG in experimental pigs., J. Květina, I. Tachecí, M. Pavlík, M. Kopáčová, S. Rejchrt, T. Douda, M. Kuneš, J. Bureš., and Obsahuje bibliografii
Modern methods of monitoring landslides are based on observations of both: direct surveying (GNSS, electronic tachymetry, geometric levelling) and remote sensing (terrestrial and aerial photogrammetry, laser scanning, interferometry), as well as surface and subsurface geotechnical observations (e.g. inclinometers, extensometers, piezometers, etc.). Due to the high cost of installation of these devices and its measurement, the implementations of these methods are usually used on well-define d objects, with established landslide activity and high risk to people’s lives. The main objective of the project was to design, create and do practical tests of simple and inexpensive measurement devices, which detect first symptoms of a potential landslide movements and alert of an existing threat. These devices would be some kind of an early warning system that would register the occurrence of the first movements of the surface layers of soil, which would be a signal to start of geodetic and geotechnical monitoring of potential landslides., Bartłomiej Ćmielewski, Bernard Kontny and Kazimierz Ćmielewski., and Obsahuje bibliografické odkazy
At present, chlorophyll meters are widely used for a quick and nondestructive estimate of chlorophyll (Chl) contents in plant leaves. Chl meters allow to estimate the Chl content in relative units - the Chl index (CI). However, using such meters, one can face a problem of converting CI into absolute values of the pigment content and comparing data acquired with different devices and for different plant species. Many Chl meters (SPAD-502, CL-01, CCM-200) demonstrated a high degree of correlation between the CI and the absolute pigment content. A number of formulas have been deduced for different plant species to convert the CI into the absolute value of the photosynthetic pigment content. However, such data have not been yet acquired for the atLEAF+ Chl meter. The purpose of the present study was to assess the applicability of the atLEAF+ Chl meter for estimating the Chl content. A significant species-specific exponential relationships between the atLEAF value (corresponding to CI) and extractable Chl a, Chl b, Chl (a+b) for Calamus dioicus and Cleistanthus sp. were shown. The correlations between the atLEAF values and the content of Chl a, Chl b, and Chl (a+b) per unit of leaf area was stronger than that per unit of dry leaf mass. The atLEAF value- Chl b correlation was weaker than that of atLEAF value-Chl a and atLEAF value-Chl (a+b) correlations. The influence of light conditions (Chl a/b ratio) on the atLEAF value has been also shown. The obtained results indicated that the atLEAF+ Chl meter is a cheap and convenient tool for a quick nondestructive estimate of the Chl content, if properly calibrated, and can be used for this purpose along with other Chl meters., E. V. Novichonok, A. O. Novichonok, J. A. Kurbatova, E. F. Markovskaya., and Obsahuje seznam literatury
The pilot study deals with the clinical results of the regenerative therapeutical method in intraosseous periodontal defects done by surgical augmentation procedure using a material of a new generation prepared on the basis of a bioactive glass. A group of 10 individuals were treated properly for chronic periodontitis. Some of treated infraalveolar periodontal lesions, both periodontal pockets and interradicular defects, persisted or recurred in posterior teeth. In order to eliminate them they were indicated for advanced periodontal surgery or regenerative surgical therapy stimulating healing processes of supportive tissues, mostly alveolar bone around treated teeth. Relevant clinical parameters, i. e. values of the pocket depth, gingival recession, and loss of attachment were evaluated before and after the periodontal surgery and compared with each other. The therapy led to significant improvement of clinical parameters in terms of the reduction of pocket depth and loss of attachment values. and R. Slezák, V. Paulusová
The article analyses the extent to which the UNIDROIT Principles of International Commercial Contracts (UPICC) are used to interpret and supplement Czech contract law. Under Czech legal doctrine the UPICC are part of lex mercatoria and not considered as a generally binding set of legal rules. However, contracting parties are free to make them part of their contract. The authors carry out a comparative analysis of selected UPICC rules and their counterparts in the Czech national law (Czech Civil Code) relating to negotiations in bad fairh, surprising terms, currency of payment, right to terminate the contract and interest for failure to pay money., Monika Pauknerová, Magdalena Pfeiffer., and Obsahuje bibliografické odkazy
This article presents a critical evaluation of the growing popularity of online social surveys for the exploration of attitudes and behaviours within higher educational institutions. More specifically this article addresses a number of key issues: the construction of representative online samples, and the presentation of the results from an institutional census constructed from an online survey with a low response rate. The improper use of statistical significance tests, and the reporting of systematic errors when quota sampling is employed in surveys is also discussed. This study compares and evaluates four recent academic surveys: (a) the Czech wave of the EUROSTUDENT IV survey fielded by SC&C, (b) A Research Survey on Academic Staff at Czech Colleges and Universities undertaken by SC&C in 2009, (c) surveys of students and (d) employees at Palacky University Olomouc undertaken by the newly established Laboratory of Social Research. This article shows that an improper interpretation of online surveys resulted in a missrepresention of the views of university students and academic staff on the state of Czech higher education and opinions concerning different tertiary education reform measures., Dan Ryšavý., and Obsahuje bibliografii a bibliografické odkazy