Reactive hyperemia (RH) in forearm muscle or skin microcirculation has been considered as a surrogate endpoint in clinical studies of cardiovascular disea e. We evaluated two potential confounders that might limit such use of RH, namely laterality of measurement and intake of non-steroidal anti-inflammatory drugs (NSAIDS). Twenty-three young non-smoking healthy adults were enrolled. In Experiment 1 (n=16), the RH elicited by 3 min of ischemia was recorded in the muscle (strain gauge plethysmography, hand excluded) and skin (laser Doppler imaging) of both forearms. In Experiment 2 (n=7), RH was determined in the dominant forearm only, one hour following oral acetylsalicylic acid (1 g) or placebo. In Experiment 1, peak RH was identical in both forearms, and so were the corresponding durations of responses. RH lasted significantly less in muscle than in skin (p=0.003), a hitherto unrecognized fact. In the skin, acetylsalicylate reduced duration (43 vs. 57.4 s for placebo, p=0.03), without affecting the peak response. In muscle, duration tended to decrease with acetylsalicylate (21.4 vs. 26.0 s with placebo, p=0.06) and the peak increase in blood flow was blunted (27.2 vs. 32.4 ml/min/100 ml tissue with placebo, p=0.003). We conclude that, when using RH as a surrogate endpoint in studies of cardiovascular disease, a confounding by laterality of measurement need not be feared, but NSAIDS may have an influence, although perhaps not on the peak response in the skin., G. Addor, A. Delachaux, B. Dischl, D. Hayoz, L. Liaudet, B. Waeber, F. Feihl., and Obsahuje bibliografii a bibliografické odkazy
This paper pursues the effect of changes in distance and vibration frequency on the vibration velocity amplitude. As an example, we used the vibrating sheet piles at the cons truction of a new multi-functional FEI building on the premises of VŠB - TU Ostrava, at 17 listopadu street. The effect of these changes is monitored both in in-situ measurements and in a simulated real-life situation. The calculation software Plaxis 2D is used for creation of numer ical models. At the close, the results from in-situ measurements are confronted with those achieved from the models., Tomáš Petřík, Eva Hrubešová and Markéta Lednická., and Obsahuje bibliografické odkazy
To determine whether PHEMA [poly(2-hydroxyethylmethacrylate)] is suitable for portal vein embolization in patients scheduled to right hepatectomy and whether it is as effective as the currently used agent (a histoacryl/lipiodol mixture). Two groups of nine patients each scheduled for extended right hepatectomy for primary or secondary hepatic tumor, had right portal vein embolization in an effort to induce future liver remnant (FLR) hypertrophy. One group had embolization with PHEMA, the other one with the histoacryl/lipiodol mixture. In all patients, embolization was performed using the right retrograde transhepatic access. Embolization was technically successful in all 18 patients, with no complication related to the embolization agent. Eight patients of either group developed FLR hypertrophy allowing extended right hepatectomy. Likewise, one patient in each group had recanalization of a portal vein branch. Hist ology showed that both embolization agents reach the periphery of portal vein branches, with PHEMA penetrating somewhat deeper into the periphery. PHEMA has been shown to be an agent suitable for embolization in the portal venous system comparable with existing embolization agent (histoacryl/lipiodol mixture)., J. H. Peregrin, R. Janoušek, D. Kautznerová, M. Oliverius, E. Sticová, M. Přádný, J. Michálek., and Obsahuje bibliografii
The aim of this study was to investigate the effects of troglitazone (TRO) - a new insulin-sensitizing agent - on some metabolic parameters in an experimental model of hypertriglyceridemia and insulin resistance, hereditary hypertriglyceridemic rats, and to compare its effects with those of vitamin E, an antioxidant agent. Three groups of the above rats were fed diets with a high content of sucrose (70 % of energy as sucrose) for four weeks. The first group was supplemented with TRO (120 mg/kg diet), the second one with vitamin E (500 mg/kg diet), and the third group served as the control. Vitamin E supplementation did not lower serum triglycerides (2.42±0.41 vs. 3.39±0.37 mmol/l, N.S.) while TRO did (1.87±0.24 vs. 3.39±0.37 mmol/l, p<0.01). Neither TRO nor vitamin E influenced the serum levels of free fatty acids (FFA). Both drugs influenced the spectrum of fatty acids in serum phospholipids - TRO increased the levels of polyunsaturated fatty acids (PUFA) n-6 (36.04±1.61 vs. 19.65±1.56 mol %, p<0.001), vitamin E increased the levels of PUFA n-3 (13.30±0.87 vs. 6.79±0.87 mol %, p<0.001) and decreased the levels of saturated fatty acids (32.97±0.58 vs. 51.45±4.01 mol %, p<0.01). In conclusion, TRO lowered the level of serum triglycerides but vitamin E did not have this effect in hypertriglyceridemic rats. Compared with TRO, vitamin E had a different effect on the spectrum of fatty acids in serum phospholipids., Š. Chvojková, L. Kazdová, J. Divišová., and Obsahuje bibliografii
The correlation between baroreflex sensitivity (BRS) and the spectrum component at a frequency of 0.1 Hz of pulse intervals (PI) and systolic blood pressure (SBP) was studied. SBP and PI of 51 subjects were recorded beat-to-beat at rest (3 min), during exercise (0.5 W/kg of body weight, 9 min), and at rest (6 min) after exercise. BRS was determined by a spectral method (a modified alpha index technique). The subjects were divided into groups according to the spectral amplitude of SBP at a frequency of 0.1 Hz. The following limits of amplitude (in mm Hg) were used: very high ≥ 5.4 (VH); high 5.4 > H ≥ 3 (H); medium 3 > M ≥ 2 (M), low < 2 (L). We analyzed the relationships between 0.1 Hz variability in PI and BRS at rest, during the exercise and during recovery in subgroups VH, H, M, L. The 0.1 Hz variability of PI increased significantly with increasing BRS in each of the groups with identical 0.1 Hz variability in SBP. This relationship was shifted to the lower values of PI variability at the same BRS with a decrease in SBP variability. The primary SBP variability increased during exercise. The interrelationship between the variability of SBP, PI and BRS was identical at rest and during exercise. A causal interrelationship between the 0.1 Hz variability of SBP and PI, and BRS was shown. During exercise, the increasing primary variability in SBP due to sympathetic activation was present, but it did not change the relationship between variability in pulse intervals and BRS., N. Honzíková, A. Krtička, Z. Nováková, E. Závodná., and Obsahuje bibliografii
The absolute gravity measurements are an important tool for reliable monitoring geodynamic phenomena. Based on the experience with the absolute gravimeter FG5#215 (gravimeter of the Center for Earth Dynamics Research), the accuracy of FG5 absolute gravimeters is presented in this study. The instrumental reproducibility of this meter is characterized by the value of 0.7 μGal. Discussed are important environmental effects on gravity measurements, such as atmospheric and hydrological effects, understanding of which is necessary for correct and reliable interpretation of the repeated absolute gravity measurements in geodynamics., Vojtech Pálinkáš, Jakub Kostelecký and Jaroslav Šimek., and Obsahuje bibliografii
In Czech and Polish underground hard coal mines of the Upper Silesian Coal Basin high-energy seismic phenomena are periodically recorded, the sources of which are located ahead of the longwall. Generally, these types of tremors are rooted in very strong, thick layers of sandstone, which are subject to the deformation border. The consequences are discontinuities and cracks with a range depending on the mechanical properties of destroyed rocks: the mechanical parameters of layers. Forecasting methods, developed in the Central Mining Institute, for stress concentration, seismic energy, fault zone and range, together with methods of rock fracturing using liquid or explosives, ,allow precise identification of suitable locations for controlled fracturing of rock mass with a pre-established direction. The size and range of discontinuities have an impact on mining parameters, dependent on basic exploitation intensity and expressed by the average daily progress of the longwall face. The rockmass is locally weakened because of exploitation or technical measures of discontinuities in the roof-rock on the longwall face. To prevent rockburst, measures are needed to reduce the amount of energy accumulating in the rockmass in the area of the longwall face. Knowledge of where stress is concentrated is extremely important for the development and implementation of effective preventative methods. For many years several research centres have been working on defining the range of these areas. In this paper, basic information is presented on methods developed by Central Mining Institute and used in Polish hard coal mines for forecasting energy concentration and assessing how it can be reduced., Jan Drzewiecki and Janusz Makówka., and Obsahuje bibliografii
Uric acid is the final product of human purine metabolism. It was pointed out that this compound acts as an antioxidant and is able to react with reactive oxygen species forming allantoin. Therefore, the measurement of allantoin levels may be used for the determination of oxidative stress in humans. The aim of the study was to clarify the antioxidant effect of uric acid during intense exercise. Whole blood samples were obtained from a group of healthy subjects. Allantoin, uric acid, and malondialdehyde levels in plasma and erythrocytes were measured using a HPLC with UV/Vis detection. Statistical significant differences in allantoin and uric acid levels during short-term intense exercise were found. Immediately after intense exercise, the plasma allantoin levels increased on the average of 200 % in comparison to baseline. Plasma uric acid levels increased slowly, at an average of 20 %. On the other hand, there were no significant changes in plasma malondialdehyde. The results suggest that uric acid, important antioxidant, is probably oxidized by reactive oxygen species to allantoin. Therefore allantoin may be suitable candidate for a marker of acute oxidative stress., R. Kanďár, X. Štramová, P. Drábková, J. Křenková., and Obsahuje bibliografii
Estimating the pre- failure points for rocks during laboratory testing is not a trivial task. In this study, a new approach is introduced that utilizes change in the slope of the load-deformation curves of rock in the loading cycle for marking the onset of failure point during uniaxial test of a given rock. At each step, load-deformation data footprints of the rock under test are inspected and a decision is made whether the failure has started or not. The load-deformation data obtained from different tests of different rocks are examined including; Norite, Granite, Limestone, Sandstone, Siltstone and Marble. The computational results over 154 cored rock samples show that the proposed approach locates the onset of failure point for a given rock with an acceptable degree of accuracy., Deniz Mamurekli., and Obsahuje bibliografii
A quantitatively new analog-to-digital converter (ADC) module has been developed during 2010, in co-operation with Tedia Ltd. The module has a 28-bit final resolution and uses 32-bit arithmetic. There are two versions, with four and twelve analog inputs. The 4-input module replaces the original 21-bit version, produced until 2009. The 12-input module is intended to be deployed in small-aperture seismic arrays. The whole set consists of four 3-channel detached modules that can be interconnected with the main module using a cable of up to 100 m in length. This design increases signal-to-noise ratio (SNR) by placing the A/D part as close to the seismograph as possible in order to transmit digital data for storage. All channels are sampled coherently so that all four sensors are automatically synchronised. It allows the detection of local events even though the sync-signal is absent. In other words, the 12-input module is suitable for ad-hoc field measurements even in places where there is no GPS signal. All arrays operated by the Institute of Rock Structure and Mechanics (IRSM) are going to be upgraded to use these modules and some new sites will also be set-up with this innovative equipment (e.g. Lazy in Western Bohemia and Dobrá Voda in Slovakia)., Milan Brož and Jaroslav Štrunc., and Obsahuje bibliografii