Soil hydraulic conductivity is a key parameter to predict water flow through the soil profile. We have developed an automatic minidisk infiltrometer (AMI) to enable easy measurement of unsaturated hydraulic conductivity using the tension infiltrometer method in the field. AMI senses the cumulative infiltration by recording change in buoyancy force acting on a vertical solid bar fixed in the reservoir tube of the infiltrometer. Performance of the instrument was tested in the laboratory and in two contrasting catchments at three sites with different land use. Hydraulic conductivities determined using AMI were compared with earlier manually taken readings. The results of laboratory testing demonstrated high accuracy and robustness of the AMI measurement. Field testing of AMI proved the suitability of the instrument for use in the determination of sorptivity and near saturated hydraulic conductivity.
Different chromosomal races of the Nannospalax ehrenbergi (Nehring, 1898) complex inhabit south-eastern Turkey. In spite of the fairly restricted range of this mole rat in Turkey, no less than eight different karyotypes have been reported so far. These differ in diploid number (range from 52 to 58), fundamental number of chromosomal arms (ranging between 72 and 90) and in the fundamental number of autosomal arms (ranging from 68 to 86). The most common chromosomal form is 2n = 52, NF = 76, NFa = 72, which is known from Southeast Anatolia. In this paper I report on a new N. ehrenbergi karyotype from south-eastern Turkey. In 18 specimens investigated, the diploid number of chromosomes was 2n = 56, NF = 66, and NFa = 62. The karyotype consisted of 4 pairs of metacentrics/submetacentrics and 23 pairs of acrocentrics. The X chromosome was medium-sized submetacentric and the Y chromosome was small acrocentric. This chromosomal set is different to the most common Turkish N. ehrenbergi karyotype (2n = 52) and also differs markedly from the known chromosomal forms from Gaziantep (2n = 56, NF = 82, NFa = 78) and Tarsus (2n = 56, NF = 72, NFa = 68 ).
Pulse dye densitometry (PDD) enables the evaluation of hemodynamic state as well as liver function. A repeated examination, even after a short pause (or under stress condition), enables to follow safely the dynamics of liver pathology. From presented parameters we have evaluated as reliable the C5-clearance, an expression of equilibrium state in the two compartment liver system. Furthermore, T-index expresses ratio of C5 value to cardiac output, it is a sensitive indicator of the blood pole, i.e. sinusoidal uptake, which is in very good correlation with staging of hepatopathies. The isolated h constant in correlation to T-index is valuable For functional grading. The Japanese automatic analyzer of indocyanine green (ICG) dilution and elimination curves, after incorporation of a two compartment mathematical mode l, becomes more useful for complex hepatological diagnostics. Non-invasive PDD is becoming of uppermost importance to clinic al interest, yielding comparable results as other complicated and invasive examinations and may be, therefore, repeated in short time intervals for different indications with minimal stress of examined patient., J. A. Tichý ... [et al.]., and Obsahuje seznam literatury
We present new explicit criteria for exponential stability of general linear neutral time-varying differential systems. Particularly, our results give extensions of the well-known stability criteria reported in \cite{Bel99,Li88} to linear neutral time-varying differential systems with distributed delays.
The paper deals with a new criterion of evaluating the disintegration by blasting operations using cylindrical as well as concentrated charges in quarries and undergrounds. The results of our research show that for seismic safety determination of blasting, the maximum of concentrated charge blasting in one delay stage is not critical, but decisive is the explosion of the total mass of blasting material., Blažej Pandula and Kamila Jelšovská., and Obsahuje bibliografické odkazy
We studied amoebae associated with nodular gill disease (NGD) outbreaks in rainbow trout Oncorhynchus mykiss (Walbaum) in fish farms in South-Western Germany. Gills of 12 diseased rainbow trout were examined in fresh, by isolation attempts, histologically and using in situ hybridisation (ISH). A total of nine amoeba strains of the genera Acanthamoeba (1), Hartmannella (2), Naegleria (1), Protacanthamoeba (1) and Vannella (4) were isolated and determined using light microscopical, ultrastructural and molecular methods. Specific molecular probes designed from the SSU rDNA sequences of individual amoeba strains were used for non-radioactive ISH in histological sections. Association of Naegleria sp. with NGD and a direct ISH proof of Naegleria trophozoites attached to hyperplastic gill epithelium are novel findings, expanding the number of possible agents of NGD and supporting the hypothesis on multicausal aetiology of this disease.
Recent examinations of newly obtained materials of dracunculoid nematodes (Dracunculoidea) parasitizing marine fishes off New Caledonia, South Pacific, revealed the presence of several nematodes of the genera Philometra Costa, 1845 (Philometridae) and Ichthyofilaria Yamaguti, 1935 (Guyanemidae), including the following four new species: Philometra priacanthi sp. n. (males) from the gonads of Priacanthus hamrur (Forsskål) (Priacanthidae), Philometra tenuicauda sp. n. (male and mature and gravid females) from the gonads of Lagocephalus sceleratus (Gmelin) (Tetraodontidae), Philometra dentigubernaculata sp. n. (males) from the oculo-orbit of Tylosurus crocodilus (Péron et Lesueur) (Belonidae), and Ichthyofilaria novaecaledoniensis sp. n. (subgravid female) from the musculature of Hoplichthys citrinus Gilbert (Hoplichthyidae). The new species are characterized mainly by the length and structure of spicules and the gubernaculum, body size, location in the host and by the type of hosts. In addition, the findings of Philometra lethrini Moravec et Justine, 2008 from the gonads of Lethrinus miniatus (Forster) and L. variegatus Valenciennes (both Lethrinidae) represent new host records for this parasite; for the first time, its subgravid females were found to be up to 350 mm long. The occurrence of Philometra ocularis Moravec, Ogawa, Suzuki, Miyazaki et Donai, 2002 in the oculo-orbit of Epinephelus areolatus (Forsskål) (Serranidae) off New Caledonia was confirmed.
The original description of Myxobolus longisporus Nie et Li, 1992, the species infecting gills of Cyprinus carpio haematopterus L., is supplemented with new data on the spore morphology and pathogenicity. Spores are elongate pyriform with pointed anterior end, 15.7 (15.5-16.5) µm long, 6.7 (6-8) µm wide and 5.5 µm thick. Sutural ridge is straight and narrow. Mucus envelope is lacking. Two equal-sized elongate pyriform polar capsules are 8.5 µm long and 2.5 µm wide with convergent long axes. Polar filament coiled perpendicularly to the long axis of the capsule makes 9 (8-10) turns. Posterior end of polar capsules exceeds mid-spore by 15-20%. Cyst-like plasmodia are localised in the gill secondary lamellae. The infection is described in adult big host specimens. Gross lesions manifested as dark red colouration of gill tissues were restricted to the ventral part of the first gill arches. Remarkable site specificity (apical part of secondary lamellae) was observed in the course of development of microscopic lesions. M. longisporus is characterised also on the molecular level using sequences of SSU rRNA gene. Phylogenetic analysis based on these sequences has allowed clearer phylogenetic relationships to be established with other species of the genus Myxobolus sequenced to date.
A myxosporean species found to develop in the liver of 10 out of 24 common shrews, Sorex araneus L., caught in South Bohemia, Czech Republic, was identified as Soricimyxum fegati Prunescu, Prunescu, Pucek et Lom, 2007, the unique representative of the genus and the first myxosporean species known to develop from plasmodia to spores in a terrestrial mammal. The original description of this species, based on fixed material, is supplemented with new data based on fresh material and with partial sequence of SSU rDNA (GenBank Acc. No. EU232760). Phylogenetic analysis of SSU rDNA revealed that S. fegati is closely related to myxosporeans infecting gall bladders of freshwater fish.