Causes of early hypoperfusion after subarachnoid hemorrhage (SAH) include intracranial hypertension as well as vasoconstriction. The aim of the study was to assess the effect of intracerebroventricular (ICV) administration of sodium nitroprusside (SNP) on early hypoperfusion after SAH. Male Wistar rats (220-240 g) were used, SAH group received 250μ
l of fresh autologous arterial blood into the prechiasmatic cistern; sham
-operated animals received 250μl of isotonic solution. Therapeutic intervention: ICV administration of 10μg SNP; 5μl 5 % glucose (SNP vehicle) and untreated control. Brain perfusion and invasive blood pressure were monitored for 30 min during and after induction of SAH. Despite SNP caused increase of perfusion in sham-operated animals, no response was observed in half of SAH animals. The other half developed hypotension accompanied by brain hypoperfusion. There was no difference between brain perfusion in SNP-treated, glucose-treated and
untreated SAH animals during the monitored period. We did not
observe expected beneficial effect of ICV administration of SNP after SAH. Moreover, half of the SNP-treated animals developed serious hypotension which led to brain hypoperfusion. This is the important finding showing that this is not the option for early management in patient after SAH.
The question was addressed whether short-term (4 hour) NO deficiency, inducing an increase in blood pressure in anaesthetized dogs, does influence proteosynthesis in the myocardium and coronary arteries. A potentially positive answer was to be followed by the study of the supporting role of ornithine decarboxylase for the polyamines pathway. NG-nitro-L-arginine-methyl ester (L-NAME) (50 mg/kg per hour) was administered i.v. to inhibit NO synthase. After the first L-NAME dose diastolic blood pressure increased from 131.8 ±2.0 to 149.4 ±3.9 mm Hg (p< 0.001) and was maintained at about this level till the end of the experiment. Systolic blood pressure only increased after the first dose (from 150.8 ±1.1 to 175.0 ±5.8 mm Hg, p<0.01), returning thereafter to the control level. Similarly, the heart rate declined only after the first dose (from 190.4±5.3 to 147.6±4.5 beats/min, p<0.01). Total RNA concentrations increased in the left cardiac ventricle (LV), the left anterior descending coronary artery (LADCA) and left circumflex coronary artery (LCCA) by 15.9 ±0.7, 29.7 ±1.3 and 17.6 ±1.0%, p<0.05, respectively. The same applied to [14C]leucine incorporation (by 86.5 ±5.0, 33.5 ±2.6, 29.3±4.1 %, p<0.05, respectively). The above parameters indicated an increase of proteosynthesis in the LV myocardium and both coronary arteries LADCA and LCCA after short-term NO deficiency. Surprisingly, the ornithine decarboxylase activity in the LV myocardium decreased significantly by 40.2± 1.6 % (p<0.01) but the changes were not significant in the coronary arteries. This unexpected finding makes the role of polyamines in increasing proteosynthesis during a pressure overload due to NO deficiency questionable.
Anthracycline cardiotoxicity represents a serious risk of anticancer chemotherapy. The aim of the present pilot study was to compare the potential of both the left ventricular (LV) filling pattern evaluation and cardiac troponin T (cTnT) plasma levels determination for the early detection of daunorubicin-induced cardiotoxicity in rabbits. The echocardiographic measurements of transmitral LV inflow as well as cTnT determinations were performed weekly for 10 weeks in daunorubicin (3 mg/kg weekly) and control groups (n=5, each). Surprisingly, no significant changes in LV-filling pattern were observed through the study, most likely due to the xylazine-containing anesthesia, necessary for appropriate resolving of the E and A waves. In contrast to the echographic measurement, the dP/dt min index obtained invasively at the end of the study revealed a significant im pairment in LV relaxation, which was further supported by observed disturbances in myocardial collagen content and calcium homeostasis. However, at the same time cTnT plasma levels were progressively rising in the daunorubicin-treated animals from the 5th week (0.024±0.008 μg/l) until the end of the experiment (0.186±0.055 μg/l). Therefore, in contrast to complicated non-invasive evaluation of diastolic function, cTnT is shown to be an early and sensitive marker of anthracycline-induced cardiotoxicity in the rabbit model., M. Štěrba, T. Šimůnek, O. Popelová, A. Potáčová, M. Adamcová, Y. Mazurová, M. Holečková, V. Geršl., and Obsahuje bibliografii a bibliografické odkazy
We undertook a detailed ultrastructural investigation to gain insight into the early stages of development of the vermiform myxozoan, Buddenbrockia plumatellae Schröder, 1910 in two bryozoan hosts. Early cell complexes arise in the peritoneum after division and migration of isolated cells in the host body wall. The development of cell junctions linking the outer (mural) cells of the complex then produces a sac enclosing a mass of inner cells. Elongation to the vermiform stage (myxoworm) occurs during multiplication and reorganisation of the inner cells as a central core within the single-layered sac wall. The core cells develop into muscle and sporogonic cells separated from the mural cells by a basal lamina. Myogenesis occurs along the length of the myxoworm from cells that differentiate from the central core, and is independent of elongation. Four primary sporogonic cells maintain positions close to the basal lamina, between muscle cells, while giving rise to secondary sporogonic cells that eventually become free in the central cavity. At least some secondary sporogonic cells undergo meiosis. In view of the recent confirmation of the phylogenetic affinity of Buddenbrockia with the Cnidaria, we postulate how features observed in Buddenbrockia may be homologous with cnidarian structures. Finally we propose a new family name, Buddenbrockiidae, to replace Saccosporidae which was proposed previously in breach of the International Code of Zoological Nomenclature.
Efficient degradation of damaged D1 during the repair of PSII is carried out by a set of dedicated FtsH proteases in the thylakoid membrane. Here we investigated whether the evolution of FtsH could hold clues to the origin of oxygenic photosynthesis. A phylogenetic analysis of over 6000 FtsH protease sequences revealed that there are three major groups of FtsH proteases originating from gene duplication events in the last common ancestor of bacteria, and that the FtsH proteases involved in PSII repair form a distinct clade branching out before the divergence of FtsH proteases found in all groups of anoxygenic phototrophic bacteria. Furthermore, we showed that the phylogenetic tree of FtsH proteases in phototrophic bacteria is similar to that for Type I and Type II reaction centre proteins. We conclude that the phylogeny of FtsH proteases is consistent with an early origin of photosynthetic water oxidation chemistry., S. Shao, T. Cardona, P. J. Nixon., and Obsahuje bibliografické odkazy
Early development of the coccidium Sarcocystis muriviperae Matuschka, Heydom, Mehlhom, Abd-Al-Aal, Diesing et Bichler, 1987 is described from experimentally infected white mice fed sporocysts from naturally infected Vipera palaestinae and Coluber jugularis. Although the course of infection was similar, mice infected with the sporocysts from the first host survived an inoculum of up to 200,000 sporocysts, while others infected with the second, succumbed to inocula exceeding 40,000 sporocysts in 7-10 days post infection (p,i,). Histological and ultrastructural studies revealed merogony in the hepatocytes during days 7-10 p.i. and onset of sarcocyst development by days 19-21 p.i. The livers of infected mice are grossly enlarged and of a mottled whitish colour due to severe neutrophil inflammatory infiltration, apparently stimulated by host cell residues or from defunct disaggregating meronts at the end of the merogony cycle. Early sarcocysts undergo a further division by endopolygeny before proceeding to division by endodyogeny.
Přirozená teologie se dá defi novat jako pokus o dokázání existence Boha pozorováním přírodního světa a užíváním rozumu, aniž by bylo bráno v potaz zjevení. Mnoho teologů si patrně myslí, že rané luteránství úplně popíralo možnost přirozené teologie, a opírají se v tom o názor samotného Luthera, podle nějž lidská přirozenost byla bytostně porušena hříchem a může se naučit vědět o Bohu pouze vírou. Ani Luther, ani jeho následovníci ovšem nebyli přesvědčeni, že je třeba zcela zavrhnout přirozenou teologii. Luther si je jist, že „všichni lidé přirozeně chápou a uznávají, že Bůh je nějakým druhem dobročinné božské moci.“ Přirozené vědění získávané rozumem je porušené hříchem a je pouze „zákonným“ věděním, ale toto vědění přesto odhaluje existenci Boha a vede nás k hledání spasitelského vědění, jehož může být dosaženo vírou poskytnutou Bohem., Natural theology can be defined as an attempt of proving the existence of God through the observation of the natural world and the use of reason, without appealing to divine revelation. Many theologians seem to think that early Lutheranism completely rejected the possibility of natural theology, based largely on the view of Luther himself that the human nature has been totally corrupted by sin and can only learn to know God through faith. It was, however, neither the understanding of Luther nor his successors to completely dismiss natural theology. Indeed, Luther is sure that “all men naturally understand and come to the conclusion that God is some kind of beneficent divine power.” Surely, the natural knowledge acquired by reason is distorted by sin and is only “legal” knowledge, but this knowledge still reveals the existence of God and leads us to look for the saving knowledge that can only be attained by faith bestowed by God., and Juuso Loikkanen.
This study concerns glazed objects from two major centres in Silesia: Wrocław and Opole. All the glazed items from layers dated from the late 10th to the first half of the 13th century are appraised, i.e. ceramic and stone Easter eggs, knobbed rattles, ‘stars’ and pottery. Each category of artefacts is appraised separately as they differ from each other in terms of the production technique and presumably their provenance. Therefore, the locations of the workshops that produced these items is considered. Based on the collections from Wrocław and Opole, a different frequency of individual glazed items is observed, which is related to their specific distribution and most likely the different role of both centres. A separate issue is determining the value and social function of the glazed vessels, Easter eggs and rattles. In contrast, less emphasis is placed on their symbolic function, as this issue has been widely discussed by other researchers.