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942. Maternal and neonatal effects of remifentanil in women undergoing cesarean section in relation to ABCB1 and OPRM1 polymorphisms
- Creator:
- Bakhouche, H., Pavlína Nosková, Svetlik, S., Bartosova, O., Jitka Ulrichová, Kubatova, J., Marusicova, P., Antonín Pařízek, Blaha, J., and Ondřej Slanař
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- Type:
- article, články, model:article, and TEXT
- Subject:
- Fyziologie člověka a srovnávací fyziologie, farmakogenetika, opioidy, polymorfismus, pharmacogenetics, opioids, polymorphism, remifentanil, 14, and 612
- Language:
- English
- Description:
- The aim of our study was to evaluate possible effect of ABCB1, and OPRM1 polymorphisms on the efficacy and safety of remifentanil in women undergoing elective cesarean section under general anesthesia. Women received remifentanil (1 μg/kg i.v.) 30 s prior to the induction to standardized general anesthesia. The ABCB1 (rs2032582, rs1045642) and OPRM1 (rs1799971) polymorphisms were analyzed from maternal peripheral blood. The basal hemodynamic and demographic parameters in the study population (n=54) were similar in all the subgroups. The median ± SD increase of systolic blood pressure at 5 min from the baseline was practically completely abolished in homozygous carriers of ABCB1 variants in comparison with wildtype subjects -2.67±25.0 vs. 16.57±15.7 mm Hg, p<0.05 for rs2032582, and 2.00±23.9 vs. 22.13±16.8 mm Hg, p<0.05, for rs1045642, respectively. While no neonate belonging to ABCB1 wild-type homozygous or OPRM1 variant carrying mothers needed any resuscitative measure, 10.5 % of the neonates belonging to OPRM1 wild-type homozygous mothers received resuscitative support similarly as 11.1 %, and 12.5 % of neonates of mothers carrying variants of rs2032582, and rs1045642, respectively. Decreased stabilizing effects of remifentanil on maternal hemodynamics has been observed in ABCB1 wild type mothers, while the adaptation of the neonates was clinically worse in OPRM1 wild type, and ABCB1 variant allele carriers., H. Bakhouche, P. Noskova, S. Svetlik, O. Bartosova, J. Ulrichova, J. Kubatova, P. Marusicova, A. Parizek, J. Blaha, O. Slanar., and Obsahuje bibliografii
- Rights:
- http://creativecommons.org/publicdomain/mark/1.0/ and policy:public
943. Mathematical model indicates nonlinearity of noradrenaline effect on rat renal artery
- Creator:
- Ďurišová, M., Ladislav Dedík, Viera Kristová, and Vojtko, R.
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- Type:
- article, články, model:article, and TEXT
- Subject:
- Experimentální medicína, fyziologie, digitální zpracování obrazu, výpočetní metody, physiology, digital image processing, computational methods, sensitivity parameter, mean effect time, effect regression, 14, and 616-092
- Language:
- English
- Description:
- The aim of this work is to present the efficacy of a previously introduced computational procedure, developed for evaluation of vascular responsiveness. On this reason, as an example a common study of noradrenaline (NA) effect on a rat renal artery under in vitro conditions was arbitrarily selected. The response of the arterial segment to NA doses (0.1-10 μg) was digitally recorded on a PC and employed to develop mathematical model of NA effect. Using the model, the following NA effect variables were determined: the vessel sensitivity parameter, mean effect time and rate constant, respectively, characterizing the effect intensity, duration, and regression and also classic response variables: the maximal effect and time of the maximal effect. The two-way analysis of variance followed by Bonferroni’s test revealed a significant influence of the increasing NA dose on the vessel sensitivity parameter and mean effect time. These findings indicated nonlinearity of processes underlying NA effect on the rat renal artery over the given range of NA doses. The procedure exemplified has the potential for use as an effective adjunct to routine studies of vascular responsiveness as it enables the extraction of meaningful information which cannot by obtained by common manual evaluation procedures., M. Ďurišová, L. Dedík, V. Kristová, R. Vojtko., and Obsahuje bibliografii a bibliografické odkazy
- Rights:
- http://creativecommons.org/licenses/by-nc-sa/4.0/ and policy:public
944. Mathematical model of the electromechanical heart contractile system - regulatory subsystem physiological considerations
- Creator:
- Mikuláš Mlček, Neumann, J., Otomar Kittnar, and Novák, V.
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- Type:
- article, články, model:article, and TEXT
- Subject:
- Fyziologie člověka a srovnávací fyziologie, fyziologie, physiology, myocardial contraction, excitation-contraction coupling, simulations, 14, and 612
- Language:
- English
- Description:
- The biochemical model of excitation-contraction coupling in cardiomyocyte is presented and the validity of simulations of both physiological and pathological processes is discussed. The model of regulatory and actomyosin subsystems, even if it is rather simple in its regulatory subunit, gives results well consistent with experimental data. Specifically, intracellular free calcium levels ([Ca2+]i) were computed under various states of sarcoendoplasmic reticular Ca2+-ATPase (SERCA2) and compared to experimental findings. Computed results reproduced well both the increase in resting [Ca2+]i level and the attenuation of [Ca2+]i decline commonly observed in heart failure. Thus the computational simulations could help to identify core relations in studied systems by comparing results obtained using similar models of various complexities., M. Mlček, J. Neumann, O. Kittnar, V. Novák., and Obsahuje bibliografii
- Rights:
- http://creativecommons.org/licenses/by-nc-sa/4.0/ and policy:public
945. May alcohol-induced increase of HDL be considered as atheroprotective?
- Creator:
- Ivana K. Lesná, Pavel Suchánek, Petr Stávek, and Rudolf Poledne
- Format:
- Type:
- article, články, model:article, and TEXT
- Subject:
- Fyziologie člověka a srovnávací fyziologie, lipoproteiny, lipoproteins, reverse cholesterol transport, cholesterol efflux, alcohol consumption, 14, and 612
- Language:
- English
- Description:
- It is well known that the consumption of moderate doses of alcohol leads to the increase of HDL-cholesterol (HDL-C). Atheroprotectivity of HDL particles is based primarily on their role in reverse cholesterol transport (RCT). In the study with a crossover design 13 male volunteers were studied in two different regimens: i) drinking of 36 g alcohol daily and ii) drinking only non-alcoholic beverages, to test whether alcohol-induced increase of HDL cholesterol can affect cholesterol efflux (CHE) from cell culture of labeled human macrophages. Alcohol consumption induced significant (p<0.05) increases of HDL cholesterol from 1.25±0.32 to 1.34±0.38 mmol/l and Apo A1 from 1.34±0.16 to 1.44±0.19 g/l. These changes were combined with a slight increase of cholesterol efflux from 13.8±2.15 to 14.9±1.85 % (p=0.059). There were significant correlations between individual changes of HDL-C and Apo A1 concentrations and individual changes of CHE (0.51 and 0.60, respectively). In conclusion, moderate alcohol consumption changes the capacity of plasma to induce CHE only at a border line significance., I. Králová Lesná ... [et al.]., and Obsahuje bibliografii a bibliografické odkazy
- Rights:
- http://creativecommons.org/licenses/by-nc-sa/4.0/ and policy:public
946. May circulating steroids reveal a predisposition to intrahepatic cholestasis of pregnancy in non-pregnant women?
- Creator:
- Patrik Šimják, Miroslav Hill, Antonín Pařízek, Libor Vítek, Marta Velíková, Michaela Dušková, Radmila Kancheva, Josef Bulant, Michal Koucký, Zuzana Kokrdová, Karolína Adamcová, Černý, A, Zdeněk Hájek, and Luboslav Stárka
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- Type:
- model:article and TEXT
- Subject:
- metabolismus steroidů, predikce, steroid metabolism, prediction, intrahepatic cholestasis of pregnancy, liver enzymes, gas chromatography-mass spectrometry, 14, and 612
- Language:
- English
- Description:
- Intrahepatic cholestasis of pregnancy (ICP) is a frequent liver disorder, mostly occurring in the third trimester. ICP is not harmful to the mothers but threatens the fetus. The authors evaluated steroid alterations in maternal and mixed umbilical blood to elucidate their role in the ICP development. Ten women with ICP were included in the study. Steroids in the maternal blood were measured by Gas Chromatography-Mass Spectrometry (GC-MS) (n=58) and RIA (n=5) at the diagnosis of ICP, labor, day 5 postpartum, week 3 postpartum and week 6 postpartum. The results were evaluated by ANOVA consisting of the subject factor, between subject factors ICP, gestational age at the diagnosis of ICP and gestational age at labor, withinsubject factor Stage and ICP × Stage interaction. The 17 controls were firstly examined in the week 36 of gestation. ICP patients showed reduced CYP17A1 activity in the C17,20 lyase step thus shifting the balance between the toxic conjugated pregnanediols and harmless sulfated 5α/β-reduced-17-oxo C19 steroids. Hence, more toxic metabolites originating in maternal liver from the placental pregnanes may penetrate backward to the fetal circulation. As these alterations persist in puerperium, the circulating steroids could be potentially used for predicting the predisposition to ICP even before next pregnancy., P. Šimják, M. Hill, A. Pařízek, L. Vítek, M. Velíková, M. Dušková, R. Kancheva, J. Bulant, M. Koucký, Z. Kokrdová, K. Adamcová, A. Černý, Z. Hájek, L. Stárka., and Obsahuje bibliografii
- Rights:
- http://creativecommons.org/publicdomain/mark/1.0/ and policy:public
947. MCT1 and MCT4 kinetic of mRNA expression in different tissues after aerobic exercise at maximal lactate steady state workload
- Creator:
- Araujo, G. G. de, Gobatto, C. A., Barros Manchado-Gobatto, F. de, Teixeira, L. F. M., Reis, I. G. M. dos, Caperuto, L. C., Papoti, M., Bordin, S., Cavaglieri, C. R., and Verlengia, R.
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- Type:
- article, články, model:article, and TEXT
- Subject:
- Fyziologie člověka a srovnávací fyziologie, genová exprese, plavání, gene expression, swimming, mice, lactate, 14, and 612
- Language:
- English
- Description:
- We evaluate the mRNA expression of monocarboxylate transporters 1 and 4 (MCT1 and MCT4) in skeletal muscle (soleus, red and white gastrocnemius), heart and liver tissues in mice submitted to a single bout of swimming exercise at the maximal lactate steady state workload (MLSSw). After 72 h of MLSS test, the animals were submitted to a swimming exercise session for 25 min at individual MLSSw. Tissues and muscle samples were obtained at rest (control, n=5 ), immediately (n=5 ), 5 h (n=5 ) and 10 h (n=5 ) after exercise for determination of the MCT1 and MCT4 mRNA expression (RT-PCR). The MCT1 mRNA expression in liver increased after 10 h in relation to the control, immediate and 5 h groups, but the MCT4 remained unchanged. The MCT1 mRNA expression in heart increased by 31 % after 10 h when compared to immediate, but no differences were observed in relation to the control group. No significant differences were observed for red gastrocnemius in MCT1 and MCT4 mRNA expression. However, white gastrocnemius increased MCT1 mRNA expression immediately when compared to rest, 5 and 10 h test groups. In soleus muscle, the MCT1 mRNA expression increased immediately, 5 and 10 h after exercise when compared to the control. In relation to MCT4 mRNA expression, the soleus increased immediately and 10 h after acute exercise when compared to the control group. The soleus, liver and heart were the main tissues that showed improved the MCT1 mRNA expression, indicating its important role in controlling MLSS concentration in mice., G. G. de Araujo, C. A. Gobatto, F. de Barros Manchado-Gobatto, L. F. M. Teixeira, I. G. M. dos Reis, L. C. Caperuto, M. Papoti, S. Bordin, C. R: Cavaglieri, R. Verlengia., and Obsahuje bibliografii
- Rights:
- http://creativecommons.org/licenses/by-nc-sa/4.0/ and policy:public
948. Meal test for glucose-dependent insulinotropic peptide (GIP) in obese and type 2 diabetic patients
- Creator:
- Jan Škrha, Jiřina Hilgertová, Jarolímková, M., Marie Kunešová, and Martin Hill
- Format:
- Type:
- article, články, model:article, and TEXT
- Subject:
- Fyziologie člověka a srovnávací fyziologie, diabetes mellitus, glucose-dependent insulinotropic peptide, type 2 diabetes mellitus, obese non-diabetic subjects, insulin secretion, 14, and 612
- Language:
- English
- Description:
- Glucose-dependent insulinotropic peptide (GIP) contributes to incretin effect of insulin secretion which is impaired in Type 2 diabetes mellitus. The aim of this study was to introduce a simple meal test for evaluation of GIP secretion and action and to examine GIP changes in Type 2 diabetic patients. Seventeen Type 2 diabetic patients, 10 obese non-diabetic and 17 nonobese control persons have been examined before and after 30, 60 and 90 min stimulation by meal test. Serum concentrations of insulin, C-peptide and GIP were estimated during the test. Impaired GIP secretion was found in Type 2 diabetic patients as compared with obese non-diabetic and non-obese control persons. The AUCGIP during 90 min of the meal stimulation was significantly lower in diabetic patients than in other two groups (p<0.03). Insulin concentration at 30 min was lower in diabetic than in non-diabetic persons and the GIP action was delayed. The ΔIRI/ΔGIP ratio increased during the test in diabetic patients, whereas it progressively decreased in obese and nonobese control persons. Simple meal test could demonstrate impaired GIP secretion and delayed insulin secretion in Type 2 diabetic patients as compared to obese non-diabetic and nonobese healthy control individuals., J. Škrha., and Obsahuje bibliografii a bibliografické odkazy
- Rights:
- http://creativecommons.org/licenses/by-nc-sa/4.0/ and policy:public
949. Measurement of cellular excitability by whole cell patch clamp technique
- Creator:
- Karmažínová, M. and Ľubica Lacinová
- Format:
- Type:
- article, články, model:article, and TEXT
- Subject:
- Fyziologie člověka a srovnávací fyziologie, genetika, molekulární genetika, genetics, molecular genetics, patch clamp, calcium current, activation, deactivation, inactivation, 14, and 612
- Language:
- English
- Description:
- Patch clamp method developed more than 30 years ago is widely used for investigation of cellular excitability manifested as transmembrane ionic current and/or generation of action potentials. This technique could be applied to measurement of ionic currents flowing through individual (single) ion channels or through the whole assembly of ion channels expressed in the whole cell. Whole cell configuration is more common for measurement of ion currents and the only one enabling measurement of action potentials. This method allows detailed analysis of mechanisms and structural determinants of voltagedependent gating of ion channels as well as regulation of channel activity by intracellular signaling pathways and pharmacological agents., M. Karmažínová, L'. Lacinová., and Obsahuje bibliografii a bibliografické odkazy
- Rights:
- http://creativecommons.org/licenses/by-nc-sa/4.0/ and policy:public
950. Measurement of Leptin and Insulin-like Growth Factor-I in Seminal Plasma from Different Species
- Creator:
- Lackey, B. R., Gray, S. L., and Henricks, D. M.
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- article, články, model:article, and TEXT
- Subject:
- Fyziologie člověka a srovnávací fyziologie, fyziologie člověka, human physiology, Leptin, Semen, Reproduction, Insulin-like growth factor, 14, and 612
- Language:
- English
- Description:
- The multi-functional proteins, insulin-like growth factor-I (IGF-I) and leptin were present in seminal plasma from different species. Concentrations of IGF-I in equine and porcine semen were 20 and 17.5 ng/ml, respectively. Seminal plasma concentrations of leptin were 1 ng/ml in human and 11 ng/ml in porcine samples., B. R. Lackey, S. L. Gray, D. M. Henricks., and Obsahuje bibliografii
- Rights:
- http://creativecommons.org/licenses/by-nc-sa/4.0/ and policy:public