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Start Over Creator Kubínová, L.

1. Confocal Stereology and Image Analysis: Methods for Estimating Geometrical Characteristics of Cells and Tissues from Three-Dimensional Confocal Images

Creator:
Kubínová, L., Janáček, J., Karen, P., Radochová, B., Difato, F., and Krekule, I.
Type:
article, model:article, and TEXT
Subject:
Confocal microscopy, Image analysis, Stereology, and Three dimensional reconstructions
Language:
English
Description:
A short review of confocal stereology and three-dimensional image analysis is presented, pointing out the achievements accomplished in this field by the Department of Biomathematics (Institute of Physiology, Prague). One of the methods of confocal stereology, the fakir method for surface area estimation, developed by this laboratory, is described. Methods for automatic measurement of geometrical characteristics of microscopical structures, based on 3-D image processing or surface triangulation, are discussed and compared with interactive stereological methods. Three-dimensional reconstruction programs and software implementation of stereological and digital methods as well as their practical applications are presented. The future trends are discussed.
Rights:
http://creativecommons.org/licenses/by-nc-sa/4.0/ and policy:public

2. STESYS software for computer-assisted stereology

Creator:
Karen, P., Kubínová, L., and Krekule, I.
Type:
article, model:article, and TEXT
Subject:
axon profde area, axon profile number, computer graphics, morphometry, and villus surface area
Language:
English
Description:
A software system STESYS for interactive and flexible generation of stereological test systems is described. STESYS enables to implement many of the recent unbiased stereological methods applied to biomedical research and clinical diagnosis by using a simple personal computer. Advantages of the STESYS software are illustrated by several examples of stereological measurements for estimating the number, total and mean cross-sectional area, volume and surface area.
Rights:
http://creativecommons.org/licenses/by-nc-sa/4.0/ and policy:public

3. Surface density and volume density measurements of chloroplast thylakoids in maize (Zea mays L.) under chilling conditions

Creator:
Kubínová, L. and Kutík, J.
Format:
bez média and svazek
Type:
model:article and TEXT
Subject:
appressed and non-appressed thylakoids, comparison of methods, granal and intergranal thylakoids, hybrids, stereology, temperature, and vertical sections
Language:
Multiple languages
Description:
Measurements of ultrastructural characteristics of chloroplast thylakoids are important for studies of ontogenic or ecological limitations of leaf photosynthetic functions. Most frequently, volumetric proportion of thylakoids in the chloroplast is measured; however, such measurement does not provide a direct information about the surface area of thylakoids which is most important from the functional point of view. Therefore, we adapted the stereological method using "local vertical windows" for estimating thylakoid surface area in the chloroplast volume and compared thus obtained surface density results with results of conventional volume density measurements. The methods were tested in the study of chloroplast ultrastructure in the leaves of plants of two maize (Zea mays L.) hybrid combinations, 2013×CE810 and CE704×CE810, developing in control and chilling conditions. Correlation analysis revealed a tight relationship between the granal/intergranal thylakoid surface density and volume density results, both indicating that under chilling conditions the development of the system of thylakoids in maize leaves is suppressed, while the difference is more pronounced in CE704 than in CE810 genotype, known to have a better photosynthetic performance. and L. Kubínová, J. Kutík.
Rights:
http://creativecommons.org/licenses/by-nc-sa/4.0/ and policy:public

4. The heterogeneity of structural and functional photosynthetic characteristics of mesophyll chloroplasts in various parts of mature or senescing leaf blade of two maize (Zea mays L.) genotypes

Creator:
Kutík, J., Holá, D., Vičánková, A., Šmídová, M., Kočová, M., Körnerová, M., and Kubínová, L.
Format:
bez média and svazek
Type:
model:article and TEXT
Subject:
chloroplast development, chloroplast dimensions, electron microscopy, Hill reaction activity, peripheral reticulum, photosynthesis, photosystem 1 activity, plastoglobuli, and thylakoids
Language:
Multiple languages
Description:
Differences in ultrastructural parameters of mesophyll cell (MC) chloroplasts, contents of photosynthetic pigments, and photochemical activities of isolated MC chloroplasts were studied in the basal, middle, and apical part of mature or senescing leaf blade of two maize genotypes. A distinct heterogeneity of leaf blade was observed both for structural and functional characteristics of chloroplasts. In both mature and senescing leaves the shape of MC chloroplasts changed from flat one in basal part of leaf to nearly spherical one in leaf apex. The volume density of granal thylakoids decreased from leaf base to apex in both types of leaves examined, while the amount of intergranal thylakoids increased in mature leaves but decreased in senescing leaves. The most striking heterogeneity was found for the quantity of plastoglobuli, which strongly increased with the increasing distance from leaf base. The differences in chloroplast ultrastructure were accompanied by differences in other photosynthetic characteristics. The Hill reaction activity and activity of photosystem 1 of isolated MC chloroplasts decreased from leaf base to apex in mature leaves. Apical part of senescing leaf blade was characterised by low contents of chlorophyll (Chl) a and Chl b, whereas in mature leaves, the content of Chls as well as the content of total carotenoids (Car) slightly increased from basal to apical leaf part. This was reflected also in the ratio Chl (a+b)/total Car; the ratio of Chl a/b did not significantly differ between individual parts of leaf blade. Both genotypes examined differed in the character of developmental gradient observed along whole length of leaf blade. and J. Kutík ... [et al.].
Rights:
http://creativecommons.org/licenses/by-nc-sa/4.0/ and policy:public