Tracking and sniffer dogs have been employed in a variety of law enforcement roles world-wide and are increasingly utilized to combat wildlife crimes (i.e. poaching and trafficking). I examined the K-9 counter--poaching unit based at Akagera National Park in Rwanda. I discuss motivations and reasoning for the unit's creation, and examine the challenges faced and methods used to overcome these. I also highlight successes and best practices in place at the Park. The major challenge encountered is health issues related to the parasitic protozoan Trypanosoma spp. Minor budgetary and facility challenges exist. Developing in-house solutions such as setting up a small clinic and basic veterinary training for handlers have reduced health risks and costs. Crossbreeding the original tracking dogs from Europe with local dogs from the communities around the park has been a success. Puppies bred and trained on-site offer affordable alternatives to purchasing additional dogs from abroad. The cross-bred dogs may also be better suited for working in the Akagera environment but further scientific study is needed to understand this. Additional successes include training dogs to track both on- and off-lead, allowing for a unit with multiple skill sets and more efficient, faster tracking.
Recent studies reported association of sleep-disordered breathing (SDB) with testosterone and vitamin D deficiency. Low testosterone and vitamin D levels have been linked to fatigue and excessive daytime sleepiness (EDS). However, the impact of testosterone and vitamin D deficiency on EDS in subjects with SDB remains unknown. The aim of this study was to explore the predictors of EDS in habitual snorers. Role of testosterone, and vitamin D was studied in detail. We also looked for associations between testosterone, vitamin D, and sleep-related indices. We prospectively enrolled 291 consecutive male patients with habitual snoring. Baseline clinical characteristics were recorded on admission. Standard overnight polysomnography was performed to detect SDB, and Epworth Sleepiness Scale (ESS) was used to assess EDS. Blood samples were obtained in a fasting condition in the morning after polysomnography to determine levels of testosterone and vitamin D. Respiratory disturbance index (RDI) (95 % CI: 1.004-1.024, p=0.005) and the use of antihistamines (95 % CI: 1.083-11.901, p=0.037) were the only independent variables significantly associated with EDS in binary logistic regression analysis. In linear multiple regression analysis, body mass index (BMI) (Beta=-0.282, p˂0.001) and oxygen desaturation index (Beta=-0.150, p=0.043) were the only independent variables significantly associated with testosterone levels, and BMI (Beta=-0.142, p=0.016) was the only independent variable significantly associated with vitamin D. We failed to find any independent association of testosterone and vitamin D with subjectively rated EDS among habitual snorers. Our results suggest an independent association between the magnitude of nocturnal desaturation and testosterone levels., Pavel Šiarnik, Matúš Jurík, Miroslava Hardoňová, Katarína Klobučníková, Jakub Veverka, Pavol Šurda, Peter Turčáni, Branislav Kollár., and Obsahuje bibliografii
Exercise can improve the cardiovascular health. However, the mechanism contributing to its beneficial effect on elderly patients with myocardial infarction is obscure. 20-month-old male Sprague-Dawley rats were used to establish myocardial infarction (MI) model by permanent ligation of the left anterior descending coronary artery (LAD) of the heart, followed by 4-week interval exercise training on a motor-driven rodent treadmill. The cardiac function, myocardial fibrosis, apoptosis, oxidative stress, and inflammatory responses were determined by using pressure transducer catheter, polygraph physiological data acquisition system, Masson's trichrome staining, and ELISA to evaluate the impact of post-MI exercise training on MI. Western blot were performed to detect the activation of AMPK/SIRT1/PGC-1α signaling in the hearts of aged rats. Exercise training significantly improved cardiac function and reduced the cardiac fibrosis. In infarcted heart, the apoptosis, oxidative stress, and inflammation were significantly reduced after 4-week exercise training. Mechanistically, AMPK/SIRT1/PGC-1α pathway was activated in the myocardial infarction area after exercise training, which might participate in the protection of cardiac function. Exercise training improves cardiac function in MI rats through reduction of apoptosis, oxidative stress, and inflammation, which may mediate by the activation of AMPK/SIRT1/PGC-1α signaling pathway.
Exercise tolerance in patients with idiopathic pulmonary fibrosis IPF is mainly limited by mechanical constrain of ventilation and high physiologic dead space. Oxygen enriched gas inhalation seems to increase ventilatory efficiency by reduction of dead space to tidal volume ratio (VD/VT) which probably mirrors improved pulmonary capillary flow and leads to longer physical tolerance at lower level of minute ventilation. The effect is noticeable at FIO2 that can be delivered in rehabilitation purposes or daily living activities.
FeNO measurement is a validated non-invasive technique, which
is used for diagnosis and monitoring of asthma. It would be
desirable to find a reliable method to monitor allergic rhinitis (AR)
via measurement of FeNO, and/or nasal nitric oxide (nNO). The
aim of our study was the assessment of the efficacy of FeNO and
nNO as markers in AR treatment. FeNO and nNO were measured
with the portable NO analyser (NIOX MINO®) in healthy
participants and in patients with AR. The patients were examined
during the pollen season and out of it. The effect of local
corticosteroids and antihistamine therapy was observed in
patients with AR during pollen season after three weeks of
therapy. There are significant differences between FeNO and
nNO in patients with AR compared to healthy controls at all set
points of measurements. While FeNO responded well to the
treatment with both antihistamines and combined therapy, nNO
decreased only after combined therapy with antihistamines and
nasal corticosteroids. nNO monitoring alone is not a suitable
method to monitor inflammation of the upper airways in AR and
its suppression by anti-allergic treatment and should be
correlated with other markers as FeNO or symptom scores.
Cílem této práce je představit české verze metod na měření vazby v dospělosti, konkrétně metody Experiences in Close Relationships a dalších variant, které jsou z ní odvozeny, spolu s jejich základními psychometrickými vlastnostmi a specifiky. Tento přehled společně s plným zněním jednotlivých dotazníků v přílohách by měl sloužit k možnosti výběru vhodného nástroje pro různé výzkumné otázky. Informace o jednotlivých metodách, jejich výhodách a nevýhodách společně s plným zněním položek by měla přispět k sjednocení používání v českém prostředí adaptovaných metod. Tento přehled by měl také přispět k omezení dalších duplicitních překladů stejných měřících nástrojů. and The aim of this paper is to present the Czech version of the methods used to measure attachment in adulthood, specifically we are concerned with the Experiences in Close Relationship method and its derived variants together with their basic psychometric properties and specifics. With the full texts of the individual questionnaires enclosed, this overview should help researchers select the appropriate version for their research questions. The information about the individual methods, their advantages and disadvantages together with the items in full ought to contribute to the unification of the use of adapted methods and to lowering the risk of repeated translations of the same methods in the context of the Czech Republic.
An infection model for sharpsnout seabream Diplodus puntazzo (Walbaum) challenged with the myxosporean Enteromyxum leei (Diamant, Lom et Dyková, 1994), resembling the natural infection conditions, was used to evaluate the antiparasitic efficacy of a functional diet. Fish of an average weight of 12.5 ± 1.2 g were delivered either a functional (included as feed supplement at 0.3% levels) or a control extruded diet. After four weeks of administration of the experimental diets, fish were challenged with the parasites (cohabitation with infected donors; donor: recipient ratio 1 : 1). The experiment was terminated four weeks after the start of the challenge. At the end of the experiment, growth and feeding (specific growth rate and feed efficiency), as well as immunological parameters (respiratory burst activity, antibacterial activities, hemoglobin concentration, anti-protease activity and ceruloplasmin activity) were measured along with cumulative mortality and total parasitic count in the gut. No significant difference was evident with regard to growth and feeding performance, mortality, gut parasitic load or immunological parameters as the parasitical challenge significantly affected both the performance of the control and functional diet fed fish. However, there was a less prominent impact on antibacterial, anti-protease and ceruloplasmin activity in fish fed with the functional diet. Overall, the present study validated the experimental cohabitation infection model and evaluated the efficacy of a functional ingredient as an antiparasitic agent, showing some potential effects on the fish immune response.
In recent years, due to the occurrence of water shortage and drought problems, particularly in arid and semiarid regions of the world, new methods to reduce evaporation from the surface of dam reservoirs, lakes, and other waterfree surfaces are investigated. This study aimed to use hydrophobic bentonite to reduce water evaporation from water surfaces, on a laboratory scale, and field conditions for the first time. Bentonite initially became hydrophobic by stearic acid (SA). Under such conditions, hydrophobic bentonite floats on the surface of water and forms a thin coating layer. The produced hydrophobic bentonite had a contact angle of 150°, indicating its superhydrophobicity. Evaporation reduction was measured under laboratory and field conditions and it was compared to hexadecanol as the reference material. The results demonstrated that the hydrophobic bentonite efficiency under laboratory conditions was similar to that of hexadecanol and prevented water evaporation by 36%. However, under field conditions, hydrophobic bentonite and hexadecanol efficiencies were 40% and 23% to reduce evaporation for 30 days, respectively. In terms of stability, hexadecanol needed to be re-injected after three days, while hydrophobic bentonite was stable and remained on the surface for more than 100 days under laboratory conditions and for more than 15 days under field conditions without needing reinjection. This coverage with method can be used to reduce evaporation from lakes, tanks, and reservoirs of small dams.
Dams have an important role in the industrial development of countries. Irrespective of the reason for dam break, the flood can cause devastating disasters with loss of life and property especially in densely populated areas. In this study, the effects of the vegetation on the flood wave propagation in case of dam break were investigated experimentally by using the distorted physical model of Ürkmez Dam. The horizontal and vertical scales of the distorted physical model are 1/150 and 1/30, respectively. The dam break scenarios were achieved by means of a gate of rectangular and triangular shape. The results obtained from experiments performed with vegetation were compared and interpreted with those obtained from experiments at which the vegetation configuration was absent. The analysis of the experimental data showed that the presence of vegetation causes a significant decrease in water depths as the flood wave propagates to the downstream and greatly reduces its impact on the settlements. It is also revealed that dam break shape plays an important role in temporal variation of flood wave.
Joints significantly reduce rock cohesion compared to unfractured rock, but the question is what effect the possible anisotropy of the shear strength of different types of rupture has on possible subsequent failure. Both natural samples of granodiorite with natural joints and fault surfaces and gypsum models have been tested on the Matest A129 Rock shear box apparatus. The shear strength of preexisting ruptures was measured under a fixed normal stress component. The anisotropy of the shear strength of the joints and fault surfaces reached more than 60 % of maximum strength, which is a very important value for solving structural loads. The shear strength was analyzed with polar plots. The pattern of the real joints typically showed a teardrop shape with one peak of strength in a certain direction and a minimum in the opposite direction. On the contrary, striated fault surfaces are characterized by two axial directions of minimal shear strength, i.e., longitudinal and transverse, and by two axial oblique directions with maximal shear strength, so the strength distribution in the polar graph has a four-cornered shape. The study showed that the anisotropy of the shear strength of various types of ruptures is their important feature.