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Start Over Date 2015 to 2016

5462. The cytotoxic effect of chelidonium majus in vitro

Creator:
Tomečková, Vladimíra, Tkáčová, Veronika, Urban, Peter, and Stupák, Marek
Format:
electronic, electronic resource, and remote
Type:
model:article, article, Text, and TEXT
Language:
English
Description:
The effect of aqueous and ether Chelidonium majus haulms extract on cervical HeLa tumor cells, mammary adenocarcinoma MCF 7 tumor cells and acute lymphoblastic leukemia CEM tumor cells in vitro have been studied. The purpose of this research was to compare the effect of aqueous and ether Chelidonium majus haulms extract on selected tumor cells. Colorimetric MTT assay have been used for the study of the antiproliferative effect of aqueous and ether haulms extract of Chelidonium majus on cell viability in vitro. The results of the experiments have shown the cytotoxic effect of the aqueous and the ether Chelidonium majus haulms extract on the individual tumor cells. The aqueous Chelidonium majus haulms extract was the most effective on CEM cells, it was less effective on MCF 7 cells and it was the least effective on HeLa cells. The ether haulms extract of Chelidonium majus was the most effective at all of studied concentrations on CEM cells and MCF 7 cells in comparison with HeLa cells, where it was significantly effective only at the highest concentration. Aqueous and ether haulms extract of Chelidonium majus tested in vitro indicated their cytotoxic activity. Both haulms extract of Chelidonium majus were more efficient on CEM cells. It is assumed that higher antiproliferative activity of ether haulms extract of Chelidonium majus is the result of higher antiproliferative activity of lipophilic substances. The lipophilic substances pass through membrane and bind to various proteins and change their biological activity., Vladimíra Tomečková, Veronika Tkáčová, Peter Urban, Marek Stupák, and Literatura
Rights:
http://creativecommons.org/publicdomain/mark/1.0/ and policy:public

5465. The dynamics of value comovement across global equity markets

Creator:
Gupta, Mayank, Novotný, Jan, Univerzita Karlova. Centrum pro ekonomický výzkum a doktorské studium, and Národohospodářský ústav (Akademie věd ČR)
Publisher:
CERGE-EI
Format:
electronic, text, regular print, bez média, svazek, and 31 stran : ilustrace.
Type:
model:monograph and TEXT
Subject:
Finance, akcie, kapitálové trhy, stocks, capital market, 336.763.2, 336.76, (048.8), 4, and 336.7
Language:
English and Czech
Description:
Mayank Gupta, Jan Novotný., Obsahuje bibliografii a bibliografické odkazy, and České a anglické resumé
Rights:
http://creativecommons.org/licenses/by-nc-sa/4.0/ and policy:public

5466. The effect of melatonin on proliferation of primary porcine cells expressing mutated Huntingtin

Creator:
Rausová, Petra, Valášek, Ján, Ellederová, Zdenka, and Motlík, Jan
Format:
print, text, and regular print
Type:
model:article, article, Text, práce podpořená grantem, srovnávací studie, and TEXT
Subject:
zvířata, prasata, prasata miniaturní, modely u zvířat, zvířata geneticky modifikovaná, Huntingtonova nemoc, proliferace buněk, mutace, statistika jako téma, mikroskopie, časové faktory, poškození DNA, fibroblasty--účinky léků, kůže, melatonin--aplikace a dávkování--farmakologie--terapeutické užití, proteiny nervové tkáně--genetika, mikroskopie buněk v čase, kožní fibroblasty, proliferační křivky, and huntingtin
Language:
Czech and English
Description:
Současné studie naznačují možnou důležitou úlohu melatoninu v Huntingtonově nemoci (HN) a jeho možné terapeutické využití při léčbě této nemoci. HN je dědičné neurodegenerativní onemocnění, které doprovází snižování hladiny melatoninu s postupem onemocnění. U normálních (nenádorových) buněk působí melatonin antiapoptoticky díky svým antioxidačním vlastnostem a schopnosti zabránit aktivaci proteinu p53. Dále melatonin zvyšuje expresi BDNF (brain derived neurotrophic factor) a dalších neuroprotektivních faktorů. Cílem této studie bylo stanovit netoxickou dávku melatoninu pro primární kožní fibroblasty izolované z transgenních miniprasat pro N‑koncovou část lidského mutovaného huntingtinu (TgHD) a popsat efekt tohoto ošetření na tyto buňky vystavené genotoxickému stresu. Buňky byly kultivovány v médiu obohaceném různými dávkami melatoninu. Analýzou proliferačních křivek získaných mikroskopováním živých buněk v pravidelných časových intervalech jsme stanovili efekt různých koncentrací melatoninu.Ukázali jsme, že vyšší dávky melatoninu jsou pro primární prasečí buňky toxické. Je zajímavé, že TgHD buňky byly oproti kontrolním buňkám více citlivé k tímto dávkám melatoninu. Stanovili jsme efektivní dávku melatoninu a současně jsme ukázali její efekt na proliferaci u buněk vystavených genotoxickému stresu. Klíčová slova: Huntingtonova choroba – melatonin – mikroskopie buněk v čase – miniprasečí model –proliferační křivky – kožní fibroblasty Autoři deklarují, že v souvislosti s předmětem studie nemají žádné komerční zájmy. Redakční rada potvrzuje, že rukopis práce splnil ICMJE kritéria pro publikace zasílané do biomedicínských časopisů., According to the recent studies, melatonin might play an important role in Huntington’s disease (HD) and act as a novel therapeutic approach in the treatment of the disease. HD, the inherited neurodegenerative disorder, is accompanied by gradual melatonin reduction as it progresses. Melatonin in normal cells (non‑tumor) has the anti‑apoptotic ability due to its antioxidant property and its ability to prevent the activation of p53. Furthermore, melatonin increases the expression of BDNF (brain derived neurotrophic factor) and other neuroprotective factors. The aim of this study was to evaluate the nontoxic dose of melatonin for primary skin fibroblasts isolated from minipigs transgenic for the N‑terminal part of human mutated huntingtin (TgHD), and the effect of melatonin treatment to these cells exposed to genotoxic stress. Cells were cultured in medium supplemented with different doses of melatonin. Using time lapse microscopy, we estimated the effect of decreasing melatonin concentrations by analyzing the proliferation curves. We show that higher doses of melatonin are toxic for primary porcine fibroblasts. Interestingly, TgHD cells were more sensitive to these doses of melatonin treatment than wild type cells. We evaluated the effective dose of melatonin and demonstrated its rescue proliferative effect on porcine primary cells exposed to genotoxic stress., and P. Rausova, J. Valasek, Z. Ellederová, J. Motlik
Rights:
http://creativecommons.org/publicdomain/mark/1.0/ and policy:public

5467. The effects of a simpler criminal procedure on criminal case outcomes: evidence from Czech district-level data

Creator:
Dušek, Libor
Publisher:
CERGE-EI
Format:
electronic, svazek, and 36 stran
Type:
model:monograph and TEXT
Subject:
Kombinatorika. Teorie grafů. Matematická statistika. Operační výzkum. Matematické modelování, trestní řízení, trestní politika, právní reformy, modelování a simulace, criminal procedure, criminal policy, legal reforms, modeling and simulation, Česko, Czechia, 343.13, 351.078:343.85, 34.047, 519.86/.87, (437.3), (048.8), 13, and 519.1/.8
Language:
English and Czech
Description:
Libor Dušek., Obsahuje bibliografii a bibliografické odkazy, and Český a anglický abstrakt
Rights:
http://creativecommons.org/licenses/by-nc-sa/4.0/ and policy:public

5468. The evaluation of the potency of newly developed oximes (K727, K733) and Trimedoxime to counteract acute neurotoxic effects of tabun in rats

Creator:
Kassa, Jiří, Hatlapatková, Jana, and Žďárová, Jana
Format:
print, text, and regular print
Type:
model:article, article, Text, časopisecké články, práce podpořená grantem, and TEXT
Subject:
zvířata, atropin--farmakologie, inhibitory cholinesteráz--toxicita, cholinesterasové reaktivátory--farmakologie, mužské pohlaví, antagonisté muskarinových receptorů--farmakologie, nervový systém--účinky léků, neuroprotektivní látky--farmakologie, neurotoxické syndromy--farmakoterapie--etiologie, otrava organofosfáty--farmakoterapie--etiologie, organofosfáty--toxicita, oximy--farmakologie, pyridinové sloučeniny--farmakologie, krysy, potkani Wistar, and trimedoxim--farmakologie
Language:
English
Description:
AIM: The ability of two newly developed oximes (K727, K733) to reduce tabun-induced acute neurotoxic signs and symptoms was evaluated and compared with currently available trimedoxime in rats. METHODS: The neuroprotective effects of the oximes studied combined with atropine on Wistar rats poisoned with tabun at a lethal dose (380 µg/kg i.m.; 90% of LD50 value) were evaluated. Tabun-induced neurotoxicity was monitored by the functional observational battery consisting of 38 measurements of sensory, motor and autonomic nervous functions at 2 hours following tabun challenge. RESULTS: All tested oximes combined with atropine enable tabun-poisoned rats to survive till the end of experiment. Both newly developed oximes (K727, K733) combined with atropine were able to decrease tabun-induced neurotoxicity in the case of lethal poisoning although they did not eliminate all tabun-induced acute neurotoxic signs and symptoms. CONCLUSION: The ability of both novel bispyridinium oximes to decrease tabun-induced acute neurotoxicity was slightly lower than that of trimedoxime. Therefore, the newly developed oximes are not suitable for the replacement of commonly used oximes such as trimedoxime in the treatment of acute tabun poisonings. and J. Kassa, J. Hatlapatková, J. Žďárová Karasová
Rights:
http://creativecommons.org/publicdomain/mark/1.0/ and policy:public

5469. The Franconian Dictionary

Creator:
König, Almut, Klepsch, Alfred, Beyschlag, Siegfried, Habermann, Mechthild, Werner, Ottmar, Straßner, Erich, Wagner, Eberhard, and Grimm, Reinhold
Publisher:
Bayerische Akademie der Wissenschaften
Type:
text and corpus
Subject:
Frankish, German, Fränkisch, Dialekt, Dialektologie, Bayern, Bavaria, Germanistik, Dialectology, dialect variation, Franconian Language, and Ostfränkisch
Language:
Frankish, German, and Mainfränkisch
Description:
The database currently contains about 1 million dialectal linguistic evidences of the project "The Franconian Dictionary" (German: Das Fränkische Wörterbuch), each of which lemmatized, annotated, and linked to the original questionnaire. The database is work in progress, so there will be more data available regularly. The Franconian Dictionary was initiated by the Munich office of the Bavarian Dictionary project, sending questionnaires for a dialect survey in Franconia. In the wake of this survey an office in Erlangen was established in 1933 (see link below for more information). During the course of 90 years thousands of volunteers helped to compile a considerable collection of vernacular examples of usage, drawn from the Bavarian districts of Upper, Middle and Lower Frankonia. For the most part they represent the East Franconian dialect, to the lesser extent also Rhine-Franconian, Swabian and North-Bavarian vernaculars. Between 2007 and 2008 a small selection of the research results was published in three editions of one printed volume by Eberhard Wagner and Alfred Klepsch: “Handwörterbuch von Bayerisch-Franken” (see link below for more information). Since 2012 the Franconian Dictionary, a project of the Bavarian Academy of Sciences and Humanities, has been entrusted to the Friedrich-Alexander-University in Erlangen and Nuremberg (FAU). The project is supervised by Prof. Dr. Mechthild Habermann, Chair of the Faculty of German Linguistics at the FAU. For detailed information, please see http://www.wbf.badw.de/en/the-project.html and http://www.wbf.badw.de/en/wbf-digital.html
Rights:
Not specified

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