Although physical exercise is known to reduce size of infarction,
incidence of ventricular arrhythmias, and to improve heart
function, molecular mechanisms of this protection are not fully
elucidated. We explored the hypothesis that voluntary running,
similar to adaptive interventions, such as ischemic or remote
preconditioning, may activate components of pro-survival (RISK)
pathway and potentially modify cell proliferation. Sprague-Dawley
adult male rats freely exercised for 23 days in cages equipped
with running wheels, while sedentary controls were housed in
standard cages. After 23 days, left ventricular (LV) myocardial
tissue samples were collected for the detection of expression and
activation of RISK proteins (WB). The day before, a marker of
cell proliferation 5-bromo-2'-deoxyuridine (BrdU) was given to all
animals to detect its incorporation into DNA of the LV cells
(ELISA). Running increased phosphorylation (activation) of Akt,
as well as the levels of PKCε and phospho-ERK1/2, whereas BrdU
incorporation into DNA was unchanged. In contrast, exercise
promoted pro-apoptotic signaling - enhanced Bax/Bcl-2 ratio and
activation of GSK-3β kinase. Results suggest that in the rat
myocardium adapted to physical load, natural cardioprotective
processes associated with physiological hypertrophy are
stimulated, while cell proliferation is not modified. Up-regulation
of pro-apoptotic markers indicates potential induction of cell
death mechanisms that might lead to maladaptation in the longterm.
Medical genetic research achieved in last decade many efforts leading to better understanding of inherited basis of human diseases. This will not be possible without the participation of patients and controls. However, the general understanding of the background and possibilities of genetic association studies is very low. It was confirmed by study of university of students. Because of the fair of misuses of the individual genetic information, significant part of participants refused the use of already donated blood samples for genetic testing but agreed with using of buccal swabs for the same analysis. To enhance the general knowledge’s of the population, leading to realistic expectations not just about genetic predictive power but also about the eventual risks in behind will be the major mission in the next years., V. Adámková ... [et al.]., and Obsahuje seznam literatury
The contribution explores the Prague origines of the first Prague and Austrian female author of the Enlightenment, Maria Anna Sager, born Rosskoschny (1719-1805). The reconstruction of the carreer of her father Anton Ferdinand Rosskoschny (1679-1734) at the Böhmische Statthalterei - he ended as "Registrator" and "Expeditor" - proves his social ambitions. On the other hand egodocuments of him conserved in the National Archives at Prague reveal the sorrows and the "stress" of the wellestablished fonctioner, not only his fear in front of the people, but also for his reputation, his family and his soul., Helga Meise., and Obsahuje bibliografické odkazy
In the War of the Austrian Succession one of the major turning points was when Maria Theresa was crowned Queen of Bohemia, because this step strengthened the power of the Houseof Habsburg in Central Europe. For people who belonged to the Reformed Church in the Kingdom of Hungary, this meant that they had to live their lives under the rule of a Catholic monarch. Debrecen was the centre of the Reformed Church and the city prepared for this political situation: pastor Mihály Komáromi H. delivered a special sermon to celebrate the coronation. In this sermon he acknowledged the fact that the Habsburgs had right to the Hungarian throne and tried to use this political advantage to improve the situation of the Reformed Church. This sermon became so popular that a manuscript was made from it and it was a popular reading in the Reformed congregations of the countryside., Ádám Hegyi., and Obsahuje bibliografické odkazy
Archeologickým výzkumem v poloze Žabník, vzdálené 500 m na JZ od obvodového valu centrální části velkomoravského hradiště v Mikulčicích, byly v r. 1968 a 1976 zachyceny pozůstatky raně středověkého pohřebiště a sídliště. Podle vertikální stratigrafie bylo pohřebiště dvoufázové a sídliště jednofázové. Rámcové datování do 9.–10. stol. vyplývá z charakteru hrobových a sídlištních nálezů, přičemž významnou roli hraje keramika. Zatímco hrobové nádoby vykazují znaky velkomoravské produkce, sídlištní keramika představuje typologicky pokročilejší zboží povelkomoravského charakteru. Navíc sídlištní keramiku reprezentuje výrazný keramicky typ se specifickými morfologickými i technologickými znaky, který v rámci mikulčické sídelní aglomerace nebyl dosud ve výraznějším zastoupení zaznamenán – byl proto pracovně označen jako keramika „typu Žabník“. and In the years 1968 and 1976, the archaeological excavations at the site of Žabník, situated app. 500 m to the south of the perimeter wall of the central part of the Great Moravian castle of Mikulčice, revealed remains of an early medieval burial ground and settlement. According to vertical stratigraphy, the burial ground consisted of two phases and the settlement of just one. The approximate dating to 9th-10th century results from the nature of the grave and settlement finds. An important role is played by pottery. While the grave vessels bear witness of Great Moravian provenience, the settlement pottery is represented by typologically more advanced products of post-Great Moravian nature. Moreover, the settlement pottery represents a distinctive ceramic type with specific morphological and technological features that has not yet been documented within the Mikulčice agglomeration in a more numerous representation – that is why it has been preliminarily designated as the Žabník pottery type.
Želatinové optické prvky vyrobené přímo žáky lze využít k naplnění cílů badatelsky orientované výuky v oblasti paprskové optiky. Článek popisuje podrobný návod na výrobu želatinových optických prvků a poskytuje námět na experimenty, při kterých žáci popíšou základní fyzikální zákonitosti paprskové optiky. Čtenář se také seznámí s výsledky předvýzkumu zabývajícího se dopadem neformálního vzdělávání na žáky., Gelatine optical elements made by pupils, can be used to achieve targets for inquiry-based science education in the field of ray optics. This article gives detailed instructions for the preparation of gelatine optical elements and provides a topic for experiments in which pupils describe the basic physical laws of ray optics. Additionally, we describe results from preliminary research dealing with the impact of extra-curricular education on pupils., Roman Chvátal., and Obsahuje bibliografické odkazy