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872. The cytotoxic effect of chelidonium majus in vitro

Creator:
Tomečková, Vladimíra, Tkáčová, Veronika, Urban, Peter, and Stupák, Marek
Format:
electronic, electronic resource, and remote
Type:
model:article, article, Text, and TEXT
Language:
English
Description:
The effect of aqueous and ether Chelidonium majus haulms extract on cervical HeLa tumor cells, mammary adenocarcinoma MCF 7 tumor cells and acute lymphoblastic leukemia CEM tumor cells in vitro have been studied. The purpose of this research was to compare the effect of aqueous and ether Chelidonium majus haulms extract on selected tumor cells. Colorimetric MTT assay have been used for the study of the antiproliferative effect of aqueous and ether haulms extract of Chelidonium majus on cell viability in vitro. The results of the experiments have shown the cytotoxic effect of the aqueous and the ether Chelidonium majus haulms extract on the individual tumor cells. The aqueous Chelidonium majus haulms extract was the most effective on CEM cells, it was less effective on MCF 7 cells and it was the least effective on HeLa cells. The ether haulms extract of Chelidonium majus was the most effective at all of studied concentrations on CEM cells and MCF 7 cells in comparison with HeLa cells, where it was significantly effective only at the highest concentration. Aqueous and ether haulms extract of Chelidonium majus tested in vitro indicated their cytotoxic activity. Both haulms extract of Chelidonium majus were more efficient on CEM cells. It is assumed that higher antiproliferative activity of ether haulms extract of Chelidonium majus is the result of higher antiproliferative activity of lipophilic substances. The lipophilic substances pass through membrane and bind to various proteins and change their biological activity., Vladimíra Tomečková, Veronika Tkáčová, Peter Urban, Marek Stupák, and Literatura
Rights:
http://creativecommons.org/publicdomain/mark/1.0/ and policy:public

873. The effect of melatonin on proliferation of primary porcine cells expressing mutated Huntingtin

Creator:
Rausová, Petra, Valášek, Ján, Ellederová, Zdenka, and Motlík, Jan
Format:
print, text, and regular print
Type:
model:article, article, Text, práce podpořená grantem, srovnávací studie, and TEXT
Subject:
zvířata, prasata, prasata miniaturní, modely u zvířat, zvířata geneticky modifikovaná, Huntingtonova nemoc, proliferace buněk, mutace, statistika jako téma, mikroskopie, časové faktory, poškození DNA, fibroblasty--účinky léků, kůže, melatonin--aplikace a dávkování--farmakologie--terapeutické užití, proteiny nervové tkáně--genetika, mikroskopie buněk v čase, kožní fibroblasty, proliferační křivky, and huntingtin
Language:
Czech and English
Description:
Současné studie naznačují možnou důležitou úlohu melatoninu v Huntingtonově nemoci (HN) a jeho možné terapeutické využití při léčbě této nemoci. HN je dědičné neurodegenerativní onemocnění, které doprovází snižování hladiny melatoninu s postupem onemocnění. U normálních (nenádorových) buněk působí melatonin antiapoptoticky díky svým antioxidačním vlastnostem a schopnosti zabránit aktivaci proteinu p53. Dále melatonin zvyšuje expresi BDNF (brain derived neurotrophic factor) a dalších neuroprotektivních faktorů. Cílem této studie bylo stanovit netoxickou dávku melatoninu pro primární kožní fibroblasty izolované z transgenních miniprasat pro N‑koncovou část lidského mutovaného huntingtinu (TgHD) a popsat efekt tohoto ošetření na tyto buňky vystavené genotoxickému stresu. Buňky byly kultivovány v médiu obohaceném různými dávkami melatoninu. Analýzou proliferačních křivek získaných mikroskopováním živých buněk v pravidelných časových intervalech jsme stanovili efekt různých koncentrací melatoninu.Ukázali jsme, že vyšší dávky melatoninu jsou pro primární prasečí buňky toxické. Je zajímavé, že TgHD buňky byly oproti kontrolním buňkám více citlivé k tímto dávkám melatoninu. Stanovili jsme efektivní dávku melatoninu a současně jsme ukázali její efekt na proliferaci u buněk vystavených genotoxickému stresu. Klíčová slova: Huntingtonova choroba – melatonin – mikroskopie buněk v čase – miniprasečí model –proliferační křivky – kožní fibroblasty Autoři deklarují, že v souvislosti s předmětem studie nemají žádné komerční zájmy. Redakční rada potvrzuje, že rukopis práce splnil ICMJE kritéria pro publikace zasílané do biomedicínských časopisů., According to the recent studies, melatonin might play an important role in Huntington’s disease (HD) and act as a novel therapeutic approach in the treatment of the disease. HD, the inherited neurodegenerative disorder, is accompanied by gradual melatonin reduction as it progresses. Melatonin in normal cells (non‑tumor) has the anti‑apoptotic ability due to its antioxidant property and its ability to prevent the activation of p53. Furthermore, melatonin increases the expression of BDNF (brain derived neurotrophic factor) and other neuroprotective factors. The aim of this study was to evaluate the nontoxic dose of melatonin for primary skin fibroblasts isolated from minipigs transgenic for the N‑terminal part of human mutated huntingtin (TgHD), and the effect of melatonin treatment to these cells exposed to genotoxic stress. Cells were cultured in medium supplemented with different doses of melatonin. Using time lapse microscopy, we estimated the effect of decreasing melatonin concentrations by analyzing the proliferation curves. We show that higher doses of melatonin are toxic for primary porcine fibroblasts. Interestingly, TgHD cells were more sensitive to these doses of melatonin treatment than wild type cells. We evaluated the effective dose of melatonin and demonstrated its rescue proliferative effect on porcine primary cells exposed to genotoxic stress., and P. Rausova, J. Valasek, Z. Ellederová, J. Motlik
Rights:
http://creativecommons.org/publicdomain/mark/1.0/ and policy:public

874. The effects of a simpler criminal procedure on criminal case outcomes: evidence from Czech district-level data

Creator:
Dušek, Libor
Publisher:
CERGE-EI
Format:
electronic, svazek, and 36 stran
Type:
model:monograph and TEXT
Subject:
Kombinatorika. Teorie grafů. Matematická statistika. Operační výzkum. Matematické modelování, trestní řízení, trestní politika, právní reformy, modelování a simulace, criminal procedure, criminal policy, legal reforms, modeling and simulation, Česko, Czechia, 343.13, 351.078:343.85, 34.047, 519.86/.87, (437.3), (048.8), 13, and 519.1/.8
Language:
English and Czech
Description:
Libor Dušek., Obsahuje bibliografii a bibliografické odkazy, and Český a anglický abstrakt
Rights:
http://creativecommons.org/licenses/by-nc-sa/4.0/ and policy:public

875. The evaluation of the potency of newly developed oximes (K727, K733) and Trimedoxime to counteract acute neurotoxic effects of tabun in rats

Creator:
Kassa, Jiří, Hatlapatková, Jana, and Žďárová, Jana
Format:
print, text, and regular print
Type:
model:article, article, Text, časopisecké články, práce podpořená grantem, and TEXT
Subject:
zvířata, atropin--farmakologie, inhibitory cholinesteráz--toxicita, cholinesterasové reaktivátory--farmakologie, mužské pohlaví, antagonisté muskarinových receptorů--farmakologie, nervový systém--účinky léků, neuroprotektivní látky--farmakologie, neurotoxické syndromy--farmakoterapie--etiologie, otrava organofosfáty--farmakoterapie--etiologie, organofosfáty--toxicita, oximy--farmakologie, pyridinové sloučeniny--farmakologie, krysy, potkani Wistar, and trimedoxim--farmakologie
Language:
English
Description:
AIM: The ability of two newly developed oximes (K727, K733) to reduce tabun-induced acute neurotoxic signs and symptoms was evaluated and compared with currently available trimedoxime in rats. METHODS: The neuroprotective effects of the oximes studied combined with atropine on Wistar rats poisoned with tabun at a lethal dose (380 µg/kg i.m.; 90% of LD50 value) were evaluated. Tabun-induced neurotoxicity was monitored by the functional observational battery consisting of 38 measurements of sensory, motor and autonomic nervous functions at 2 hours following tabun challenge. RESULTS: All tested oximes combined with atropine enable tabun-poisoned rats to survive till the end of experiment. Both newly developed oximes (K727, K733) combined with atropine were able to decrease tabun-induced neurotoxicity in the case of lethal poisoning although they did not eliminate all tabun-induced acute neurotoxic signs and symptoms. CONCLUSION: The ability of both novel bispyridinium oximes to decrease tabun-induced acute neurotoxicity was slightly lower than that of trimedoxime. Therefore, the newly developed oximes are not suitable for the replacement of commonly used oximes such as trimedoxime in the treatment of acute tabun poisonings. and J. Kassa, J. Hatlapatková, J. Žďárová Karasová
Rights:
http://creativecommons.org/publicdomain/mark/1.0/ and policy:public

877. The impact of management quality on innovation performance of firms in emerging countries

Creator:
Sidorkin, Oleg
Publisher:
CERGE-EI
Format:
electronic, bez média, svazek, and 39 stran : ilustrace.
Type:
model:monograph and TEXT
Subject:
Management. Řízení, management jakosti, inovace, quality control, innovations, 005.6, 330.341.1, (048.8), 4, and 005
Language:
English
Description:
Oleg Sidorkin., Obsahuje bibliografii a bibliografické odkazy, and born digital
Rights:
http://creativecommons.org/licenses/by-nc-sa/4.0/ and policy:public

879. The Libechov minipig as a large animal model for preclinical research in Huntington’s disease – thoughts and perspectives

Creator:
Schramke, S., Schubert, R., Frank, F., Wirsig, M., Fels, M., Kemper, N., Schuldenzucker, V., and Reilmann, R.
Format:
print, text, and regular print
Type:
model:article, article, Text, práce podpořená grantem, and TEXT
Subject:
zvířata, zvířata geneticky modifikovaná, prasata, prasata miniaturní, modely u zvířat, preklinické hodnocení léčiv, Huntingtonova nemoc, chov, bydlení zvířat, časové faktory, mozek--anatomie a histologie, velikost orgánu, experimenty na zvířatech, etika výzkumu, and sekvenční homologie
Language:
Czech and English
Description:
Large animal models to explore the safety and tolerability of novel therapeutic approaches for Huntington’s disease (HD) are in exploration to achieve higher translational reliability in future studies. Recently, a Libechov minipig has been established as one new transgenic (Tg) large animal model for HD. We here discuss the advantages and limitations in using this model in HD with regards to breeding, housing, handling, and with respect to homology to humans and ethical considerations. A group of TgHD and wild type (WT) female minipigs (n = 36) was used to gain first evidence about abovementioned aspects. It is concluded that Libechov minipigs may fulfill an important role to bridge the gap between rodents and non‑human primates in the translation to humans. and S. Schramke, R. Schubert, F. Frank, M. Wirsig, M. Fels, N. Kemper, V. Schuldenzucker, R. Reilmann
Rights:
http://creativecommons.org/publicdomain/mark/1.0/ and policy:public

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