To understand the pathogenesis of hypercholesterolemia in Prague hereditary hypercholesterolemic (PHHC) rat, we analyzed the response of hepatic transcriptome to dietary cholesterol in PHHC and control Wistar rats. Male PHHC and Wistar rats were fed chow (C), 5 % fat (palm kernel oil) (CF) or 1 % cholesterol + 5 % fat (CHOL) diet for three weeks. Hepatic transcriptome was analyzed using Affymetrix GeneChip arrays. No differences were found in the effect of both control diets (C and CF) on lipid metabolism and gene expression of 6500 genes. Therefore, these data were pooled for further analysis. Dietary cholesterol induced accumulation of cholesterol and triacylglycerols in the liver in both strains and hypercholesterolemia in PHHC rats. However, there were no differences in response of hepatic transcriptome to CHOL diet. On the other hand, several genes were found to be differently expressed between both strains independently of the diet. Two of those genes, Apof and Aldh1a7, were studied in more detail, and their role in pathogenesis of hypercholesterolemia in PHHC rats could not been corroborated. In conclusion, the hypercholesterolemia in PHHC rats is due to physiological response of hepatic transcriptome to dietary cholesterol in different genetic background., M. Vlachová, M. Heczková, M. Jirsa, R. Poledne, J. Kovář., and Obsahuje bibliografii
The objective of this study was to investigate the response of Na+/K+-ATPase of human erythrocytes to green laser irradiation. Effects of green laser light of fluences 9.5-63.3 J.cm-2 and merocyanine 540-mediated laser light treatment were studied. Isolated erythrocyte membranes (protein concentration of 1 mg/ml) were irradiated by Nd:YAG laser (532 nm, 30 mW) and then incubated in a medium with 2 mM ATP for 30 min. Activity of ATPase was determined colorimetrically by measuring the colored reaction product of liberated inorganic phosphate and malachite green at 640 nm. Contribution of Na+/K+-ATPase to overall phosphate production was determined using ouabain. A positive effect of green laser light on Na+/K+-ATPase activity was observed. The dependence of enzymatically liberated inorganic phosphate on light fluence showed a linear correlation (R2=0.96, P=0.0005) for all fluences applied (9.5-63.3 J.cm-2). On the other hand, MC 540-mediated phototreatment caused a suppression of enzyme activity.
Effects of excess Cd and Cu on the contents of total glutathione and phytochelatin (PC) were measured in roots and leaves of intact spinách piants. The total glutathione levels declined with the PCs synthesis. The kinetic data indicated that the shorter PCs were substrates for the longer PCs. PCs appear much earlier in roots than in leaves of intact spinách piants. The PC formation was stimulated most effectively by Cd, less so by Cu.
The photosynthetic parameters in leaves of three-year-old seedlings of Fraxinus rhynchophylla L. were studied under different soil water conditions and CO2 concentrations ([CO2]) with a
LI-COR 6400 portable photosynthesis system. The objective was to investigate the response of photosynthesis and stomatal conductance (gs) to various [CO2] and soil water conditions, and to understand the adaptability of F. rhynchophylla to such conditions. The results showed that the soil water content (RWC) required to maintain high photosynthetic productivity in F. rhynchophylla was 49.5-84.3%; in this range, net photosynthetic rate (PN) rose with [CO2] increasing from 500 to 1,400 μmol mol-1. Outside this RWC range, PN decreased significantly. The apparent maximum photosynthetic rate (Pmax,c) and carboxylation velocity (Vc) increased with increasing RWC and remained relatively high, when RWC was between 49.5 and 96.2%. CO2 compensation points and photorespiration rate exhibited a trend opposite to that of Pmax,c and Vc, indicating that moderate water stress was beneficial for increasing plant assimilation, decreasing photorespiration, and increasing production of photosynthates. gs declined significantly with increasing [CO2] under different water supplies, but the RWC range maintaining high gs increased. gs reached its maximum, when RWC was approximately 73% and then decreased with declining RWC. The maximal gs was found with increasing RWC. Thus, based on photosynthetic characteristics in artificial, vegetation construction in semiarid loess hill and gully area, F. rhynchophylla could be planted in habitats of low soil water content. and S. Y. Zhang ... [et al.].
The article discusses the theoretical and methodological considerations as well as the partical application of two incarnations of the rhetorical turn in socio-cultural anthropology. Rhetorical turn is understood as a linguistic and constructivist turn, which marks a substantial part of contemporary thinking in the social sciences and humanities.
This article outlines the right of asylum as a judicial institute in the early modern period, when modern states have been forming. Special attention is given to the dynamic struggle between the church asylum, as well as the church judicial system in general, and the state’s secular power.Specific attributes of the right of asylum and its evolution in time are studied from different viewpoints; understanding of the term and its content, legal continuity of the right of asylum,conditions, under which asylum was granted and the different forms of asylum. General observations and supported by specific historical examples and references.
It is argued in the article that the peaceful transition to capitalism in communist countries was not possible without the co-action of the nomenklatura, whose interest was to transform their informal access to state-owned capital into an authentic 'grand entrepreneurship'. The necessary acquisition of physical cap ital was achieved by means of mass privatisation schemes in which the nomenklatura took advantage of their social capital and information asymmetries. In the Czech case, there were three social groups competing for a position among the new entrepreneurial elite. The initially large gains of the nomenklatura gradually eroded when new businesses opened to domestic and international competition, where competitiveness depended on endowments of human (entrepreneurial) and economic capital. In the subsequent wave of ownership restructuring, initiated after 1994, the former nomenklatura was partially squeezed out of the tradable sector, which was occupied by better skilled foreign and domestic entrepreneurs. The exiting entrepreneurs converted their holdings into consumer goods, or defected to sectors less open to competition, where the alignment of social capital and bureaucracy persisted. Their position depends now on the pending reforms of public administration and the search for a more efficient social model.
Salinization is an increasing enviromental problem in ecosystems. The assessment of total dissolved solids (TDS), pH, electrical conductivity (EC), exchangeable sodium percentage (ESP), alkalinity and the concentrations of main ions makes possible to identify salinization and alkalization degree. The saltaffected soils occur in the south part and south-east part of Žitný ostrov, where the dry and mild summer climate, evaporation soil water regime and mineralized groundwater create conditions for development of the saline and alkaline soils. Five localities with highly-mineralized groundwater were monitored to evaluate salinity and alkality in the period 1989-2006. At the bottom horizons in localities 1 - 5 evaporative residues (salt content) reached value 0.1 - 0.2 %. In 2006 dry evaporative residues (salt content) was higher than 0.2 %, EC was higher than 250 mS m-1 at the bottom horizons in localities 1, 2, 4. The mentioned data allow us to state that salinization and alkalization of soils is developing, it starts from the bottom of soil horizons through middle part of the soil profile up to the top horizons. This phenomenon was observed in all investigated soils in different stages of development. and Salinizácia v ekosystémoch je stále významnejší enviromentálny problém. Stanovenie celkového množstva rozpustených látok (TDS), elektrickej vodivosti (EC), sodíkového adsorpčného pomeru (SAR) a obsahu výmenného sodíka (ESP) umožňuje identifikáciu kritického potenciálu salinizácie a degradácie pôdy. Záujmové územie bolo vymedzené vo východnej časti Žitného ostrova, kde suché a horúce klimatické podmienky spolu s vysokomineralizovanými podzemnými vodami sú príčinou translokácie solí do pôdneho profilu. Bolo monitorovaných 5 lokalít v období 1989 - 2006. Namerané údaje poukazujú na vzrastajúci obsah solí, ktorý v r. 2006 dosiahol 0,1 - 0,2 % v celom pôdnom profile vo všetkých monitorovaných lokalitách, hodnoty EC 200 - 400 mS m-1 v spodných horizontoch vytvárajú podmienky pre rozvoj zasolených pôd.
Numerous hypotheses have been proposed about the pathogenesis of the polycystic ovarian syndrome (PCOS). However, hormonal control of persistent follicles has not be enestablished. The objective of the present study was to compare the follicular structure and hormonal profiles of rats treated with the adrenocor ticotrophic hormone (ACTH) with two experimental models of PCOS. ACTH-treated animals were compared with those exposed to continuous light, those treated with estradiol valerate, and with control (in proestrous and diestrous). Serum hormone levels, histomorphometrical changes, and immunoexpression of vimentin, cytokeratins, cadherins, and proliferating cell nuclear antigen (PCNA) were examined. Treatment with ACTH resulted in an elevation of corticosterone secretion with LH reduction but without changes in ovarian morphology. Although stress (or ACTH) stimulation may be only one of pathophysiological mechanisms involved in follicular cystathogenesis in other species, we do not have important evidence to suppose that this would happen in rats., C. Bavaralle, N. R. Salvetti, G. A. Mira, J. A. Lorente, H. H. Ortega., and Obsahuje bibliografii a bibliografické odkazy
Certain liver metabolic diseases point to the presence of disturbances in glycogen deposition. Epinephrine raises the cAMP level that activates protein kinase A leading to the activation of phosphorylase and glycogen breakdown. In the present report, we sought to investigate whether NO is produced during adrenoceptor agonist-induced glycogenolysis in rat hepatocytes in cultures. Isolated glycogen rich rat hepatocytes in cultures were used. NO production (NO2-) was assessed under the effect of adrenergic agonists and adrenergic agonist/antagonist pairs, dibutyryl cyclic AMP sodium-potassium salt (db-cAMP), NO synthase (NOS) inhibitors Nω-nitro-L-arginine methyl ester (L-NAME), aminoguanidine (AG) and the NO donor S-nitroso-N-acetyl penicillamine (SNAP) . The inducible NO synthase (iNOS) mRNA was examined by the reverse transcription-polymerase chain reaction (RT-PCR). Glycogenolysis was quantified by glucose levels released into medium. The amount of glucose and NO2- released by hepatocytes was increased as a result of epinephrine, phenylephrine or db-cAMP treatments. The increase in glucose and NO2- released by epinephrine or phenylephrine was blocked or reduced by prazosin pretreatment and by NOS inhibitors aminoguanidine and L-NAME. iNOS gene expression was up-regulated by epinephrine. It can be concluded that glycogenolysis occurs through α adrenoceptor stimulation and a signaling cascade may involve NO production., J. Hodis, N. Kutinová-Canová, P. Potměšil, L. Kameníková, E. Kmoníčková, Z. Zídek, H. Farghali., and Obsahuje biblografii a bibliografické odkazy