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2. Antioxidant status and lipid peroxidation in diabetic rats under hyperbaric oxygen exposure
- Creator:
- Matsunami, T., Sato, Y., Sato, T., and Yukawa, M.
- Format:
- Type:
- article, články, model:article, and TEXT
- Subject:
- Fyziologie člověka a srovnávací fyziologie, oxidační stres, oxidative stress, hyperbaric oxygen, diabetic rats, lipid peroxidation, antioxidant enzyme, 14, and 612
- Language:
- English
- Description:
- Hyperglycemia is known to cause oxidative stress that leads mainly to enhanced production of mitochondrial reactive oxygen species (ROS). It has been demonstrated that hyperbaric oxygen (HBO) treatment also increases the formation of ROS. There are, however, no comprehensive evaluations of such oxidative effects in diabetes which requires HBO treatment. The purpose of this study is to investigate the influence of a clinically-recommended HBO treatment on glucose homeostasis and oxidative stress in rats with streptozotocin (STZ)-induced diabetes. Under the clinically-used HBO exposure protocol, the levels of blood glucose, thiobarbituric acid reactive substances (TBARS) as a lipid peroxidation marker, and the activity of superoxide dismutase (SOD) as an antioxidant enzyme marker were investigated in the erythrocytes, liver, pancreas, skeletal muscle, and brain of rats with STZ-induced diabetes. The levels of blood glucose and TBARS increased significantly (p<0.05), and the activity of SOD decreased significantly (p<0.05) in the erythrocytes and all organs of rats with diabetes subjected to HBO exposure. These results suggested that HBO exposure might boost glucose autoxidation and increase ROS production in STZ-induced diabetes as side-effects of administering HBO treatment for the first time., T. Matsunami ... [et al.]., and Obsahuje bibliografii a bibliografické odkazy
- Rights:
- http://creativecommons.org/licenses/by-nc-sa/4.0/ and policy:public
3. Cerebrolysin inhibits lipid peroxidation induced by insulin hypoglycemia in the brain and heart of mice
- Creator:
- Jitka Patočková, Miloslav Kršiak, Petr Marhol, and Eva Tůmová
- Format:
- print, bez média, and svazek
- Type:
- article, články, model:article, and TEXT
- Subject:
- Fyziologie člověka a srovnávací fyziologie, hyperglykémie, hyperglycemia, lipid peroxidation, TBARs, cerebrolysin, 14, and 612
- Language:
- English
- Description:
- As a consequence of enhanced production of oxygen free radicals, lipid peroxidation leads to the degradation of membrane lipids and disturbances of membrane permeability. Lipid peroxidation increases under stress conditions such as hypoxia, ischemia or acidosis as well as in metabolic diseases, e.g. diabetes mellitus. We have shown that subcomatous doses of insulin (6.0 IU/kg) significantly increase thiobarbituric acid reactive substances (TBARs), especially malondialdehyde (MDA) - the endproduct of lipid peroxidation, in the brain and heart of mice. In our model of insulin-induced hypoglycemia, mice were treated with the neuroprotective, peptide-containing drug Cerebrolysin (100 mg/kg b.w.). Animals were sacrificed by decapitation two or three hours after the injection of tested substance and samples were taken to determine several serum parameters (glucose, total protein, triglycerides and lactic acid) and TBARs in the brain and heart. Although Cerebrolysin was not able to affect serum parameters after subcomatous insulin injection, the drug significantly influenced lipid peroxidation. A single injection of Cerebrolysin already decreased TBARs levels in the brain and heart tissue. Presuming that an increase of TBARs reflects disturbances of the cell membrane, we have documented a promising effect of Cerebrolysin on cell integrity., J. Patočková, M. Kršiak, P. Marhol, E. Tůmová., and Obsahuje bibliografii
- Rights:
- http://creativecommons.org/licenses/by-nc-sa/4.0/ and policy:public
4. Changes in blood haemorheological parameters after submaximal exercise in trained and untrained subjects
- Creator:
- Gürcan, N., Erbas, D., Ergen, E., Bilgehan, A., Dündar, S., Aricioglu, A., and Dikmenoglu, N.
- Type:
- article, model:article, and TEXT
- Subject:
- submaximal exercise, glutathione peroxidase, lipid peroxidation, and haemorheology
- Language:
- English
- Description:
- The blood stream is affected by viscosity and many other haemorheological factors such as lipid peroxidation in the plasma and red blood cells. The aim of this study was to investigate the changes of haemorheological parameters after submaximal exercise in trained and untrained subjects. The results indicated that heart rate, lymphocyte count, erythrocyte deformability, plasma lipid peroxide levels and erythrocyte glutathione peroxidase activity are increased after submaximal exercise.
- Rights:
- http://creativecommons.org/licenses/by-nc-sa/4.0/ and policy:public
5. Could the musk compound tonalide affect physiological functions and act as an endocrine disruptor in rainbow trout?
- Creator:
- Hodkovicova, Nikola, Enevova, Vladimira, Cahova, Jana, Blahova, Jana, Siroka, Zuzana, Plhalova, Lucie, Doubkova, Veronika, Marsalek, Petr, Franc, Ales, Fiorino, Emma, Faggio, Caterina, Tichy, Frantisek, Faldyna, Martin, and Svobodova, Zdenka
- Format:
- počítač and online zdroj
- Type:
- model:article and TEXT
- Subject:
- hematology, biochemistry, histology, lipid peroxidation, and oncorhynchus mykiss
- Language:
- English
- Description:
- In the present study, the effect of polycyclic musk compound tonalide (AHTN) in two concentrations was studied in male rainbow trout (Oncorhynchus mykiss, Walbaum 1792). A feeding trial was conducted with AHTN incorporated into feed granules. One concentration was environmentally relevant (854 µg/kg); the second one was 10× higher (8699 µg/kg). The fish were fed twice a day with the amount of feed at 1 % of their body weight. After an acclimatization period, the experimental phase in duration of six weeks followed. At the end of the experiment, fish were sampled and the biometrical data were recorded. Subsequently, hematological and biochemical tests, histopathological examination, analysis of oxidative stress markers and evaluation of endocrine disruption using plasma vitellogenin were performed. In conclusion, an increase of hematocrit for both AHTN concentrations was found, but no significant changes were observed in biochemical profile. Moreover, AHTN caused lipid peroxidation in caudal kidney tissue, which was confirmed by histopathological images. The long-lasting AHTN exposure could thus be harmful for maintaining homeostasis in the rainbow trout organism. However, the vitellogenin concentration seemed not to be affected by AHTN.
- Rights:
- http://creativecommons.org/publicdomain/mark/1.0/ and policy:public
6. D-galactose induced changes in enzymatic antioxidant status in rats of different ages
- Creator:
- Hadzi-Petrushev, N., Stojkovski, V., Mitrov, D., and Mladenov, M.
- Format:
- print, bez média, and svazek
- Type:
- article, články, model:article, and TEXT
- Subject:
- Fyziologie člověka a srovnávací fyziologie, stárnutí, aging, d-galactose, senescence, antioxidant enzymes, lipid peroxidation, wistar rats, 14, and 612
- Language:
- English
- Description:
- Considering the preexisting influence of the process of natural aging on antioxidant enzymes activity and the level of lipid peroxidation, the age of the rats at which D-galactose (D-gal) treatment is started could strongly impact the development of D-gal induced senescence. To eval uate this, we subjected 1, 3 and 15 months old rats to D-gal treatment in parallel with having appropriate placebos (0.9 % saline). Our results showed elevated glutathione peroxidase (GPx) acti vity and no significant changes in superoxide dismutase (SOD), catalase (CAT) and glutathione reductase (GR) activity or malondialdehyde (MDA) levels in relation to natural aging. In mature and aged senescent livers we observed positive correlation between increased ratio R=SOD/(GPx+CAT) and increased MDA concentration. MDA levels seemed to correlate positively with the age of the animals at which D-gal treatment had started. In the case of 3 and 15 months old rats there was D-gal induced decrease in SOD and GR activity, but this effect of the treatment was not observed in 1 month old rats. Our results imply that the changes in the antioxidant enzyme activities are not only under the influence of the D-gal overload, but also depend on the developmental stage of the rats. According to our resu lts, with regard to enzymatic antioxidant capacity and the level of lipid peroxidation, the best age for induction of senescence is somewhere after the third month., N. Hadzi-Petrushev, V. Stojkovski, D. Mitrov, M. Mladenov., and Obsahuje bibliografii
- Rights:
- http://creativecommons.org/licenses/by-nc-sa/4.0/ and policy:public
7. Different degrees of lipid peroxidation in the CNS of young and adult rats exposed to short-term hypobaric hypoxia
- Creator:
- Koudelová, J. and Mourek, J.
- Type:
- article, model:article, and TEXT
- Subject:
- hypobaric hypoxia, lipid peroxidation, CNS development, and rat
- Language:
- English
- Description:
- The authors studied the effect of short-term (20 min) hypobaric hypoxia at simulated altitudes of 7000 and 9000 m on the peroxidation of lipids in the cerebral cortex, subcortical formations, medulla oblongata and cerebellum of the laboratory rat. In 5- and 21-day-old rats, increased lipoperoxidation was recorded in all the studied regions of the brain. Differences were observed in sensitivity to the degree of hypoxia. In 5-day-old rats the response to both exposures was the same, but in 21-day-old animals exposure at 7000 m stimulated peroxidation in the cerebral cortex only (at 9000 m in all the parts of the CNS examined). In 35-day-old and adult rats, changes in the malondialdehyde concentration were likewise found after exposure at 9000 m, but not in every compartment (in 35-day-old rats in the cerebral cortex and subcortical formations and in adult rats in the cerebral cortex). In young rats, 30 and 60 min after exposure to hypoxia the malondialdehyde concentration was still higher than in older animals.
- Rights:
- http://creativecommons.org/licenses/by-nc-sa/4.0/ and policy:public
8. Differential impact of acute bout of exercise on redox- and oxidative damage-related profiles between untrained subjects and amateur runners
- Creator:
- Falone, S., Mirabilio, A., Pennelli, A., Cacchio, CM., Di Baldassarre, A., Gallina, S., Passerini, A., and Amicarelli, F.
- Format:
- print, bez média, and svazek
- Type:
- article, články, model:article, and TEXT
- Subject:
- Fyziologie člověka a srovnávací fyziologie, oxidační stres, tělesná kondice, oxidative stress, physical fitness, aerobic exercise, reactive oxygen species, lipid peroxidation, 14, and 612
- Language:
- English
- Description:
- Despite the demonstrated exercise -induced increase in reactive oxygen species (ROS) production, growing epidemiological evidence indicates that habitual, moderate physical activity reduces the incidence of several oxidative stress-based diseases. This apparent paradox can be explained taking into account that ROS produced during repeated ex ercise bouts may act as mild stressors able to trigger physiological and biomolecular hormetic responses through a number of redox-sensitive transcription pathways. Unfortunately, much more limited information is available from general population-based research, which could better reflect the condition of common people interested in achieving and maintaining good fitness levels. The present work aimed at investigatin g whether and how exercise-related habits in non-professional regular runners (n=33) can affect the systemic anti-oxidative capacity, and the resting serum levels of typical lipid peroxidation-related by-products and oxidatively- damaged proteins, in comparison with untrained sedentary individuals (n=25). We also anal yzed in both groups the redox response elicited by a modified Bruce-based maximal exercise test on the same parameters. Our findings indicated that long- term regular and moderate practice of aerobic physical activity can increase antioxidant defense systems, lower the resting protein oxidation processes and reduce the immediate up- regulation of lipid-targeting oxidative stress in response to an acute bout of exercise., S. Falone, A. Mirabilio, A. Pennelli, M. Cacchio, A. Di Baldassarre, S. Gallina, A. Passerini, F. Amicarelli., and Obsahuje bibliografii
- Rights:
- http://creativecommons.org/licenses/by-nc-sa/4.0/ and policy:public
9. Effect of cisplatin, carboplatin and stobadine on lipid peroxidation of kidney homogenate and phosphatidylcholine liposomes
- Creator:
- Gergeľ, O., Mišík, V., and Ondriaš, K.
- Type:
- article, model:article, and TEXT
- Subject:
- cisplatin, carboplatin, stobadine, kidney Homogenate, liposomes, and lipid peroxidation
- Language:
- English
- Description:
- The effects of the nephrotoxic, anticancer agents cisplatin (CDI)P) and carboplatin (CBDCA), and the free radical scavenger, stobadine, were investigated on lipid peroxidation (EI’O) of rat kidney homogenates and phosphatidylcholine (PC) liposomes. Kidney homogenates were incubated in air at 37 °C for 6-48 h and lipid peroxidation was detected spectroscopically as absorbance (533 nm) of the thiobarbituric acid- malondialdchyde (TBA-MDA) complex. CDDP (0.3-10 mmol.I'1) increased LPO of the homogenate. CBDCA decreased the TBA-MDA absorbance, yet was found to interfere with MDA, TBA and/or with the TBA-MDA complex. Thus when CBDCA is involved, the TBA- MDA method for detection of LPO is not suitable. Stobadine (0.1 mmol.I'1 ar>d 1 mmol.I1) inhibited LPO either in the control homogenate and in the homogenate where peroxidation was increased by CDDP. The effect of CDDP and CBDCA on peroxidation of PC liposomes was monitored as oxygen consumption using a Clark-type oxygen electrode. CDDP increased but CBDCA decreaed the rate of oxygen consumption during the peroxidation of liposomes induced by FcSO,». The results suggest that the effects of CDDP and CBDCA on LPO may be linked with their nephrotoxicity.
- Rights:
- http://creativecommons.org/licenses/by-nc-sa/4.0/ and policy:public
10. Erythrocyte membrane fluidity and tissue lipid peroxides in female Guinea-pigs on graded vitamin C intake
- Creator:
- Tatara, M. and Ginter, E.
- Type:
- article, model:article, and TEXT
- Subject:
- membrane fluidity, erythrocytes, vitamin C, Guinea-pig, and lipid peroxidation
- Language:
- English
- Description:
- The effect of low (1 mg/animal/day), medium (10 mg/animal/day) and high (100 mg/animal/day) intake of ascorbic acid on tissue lipid peroxidation (LPO) and the physical state of erythrocyte membranes was investigated in female guinea-pigs fed a vitamin E low diet. Animals were killed after 9-11 weeks and the blood, liver, lungs, kidneys and adrenals were analysed. The LPO was estimated by the determination of malondialdehyde with HPLC. The physical state of erythrocyte membranes was determined spectrofluorometrically and expressed as fluorescence polarization of membrane lipid specific probe 1,6-diphenyl-1,3,5-hexatriene. The LPO concentrations in the liver and adrenals of the group on a low vitamin C intake were significantly increased. A significant non-linear negative correlation between C vitamin levels and LPO concentrations was found in these tissues. The fluidity of erythrocyte membranes as a measure of their structural state was significantly lower in the group with a low intake of C vitamin. It is probable that the water-soluble antioxidants, such as vitamin C, act in the plasma as primary defense against oxidative stress if the radicals are formed initially in the aqueous phase of whole blood.
- Rights:
- http://creativecommons.org/licenses/by-nc-sa/4.0/ and policy:public